ABSTRACT: Iron oxide nanoparticles were synthesized through a green synthesis method with Dalbergia sissoo leaf extract being used as a reducing/stabilizing agent. The method presented an eco-friendly, straightforward way to synthesize iron oxide nanoparticles. We explored the influence of key factors in the synthesis, including precursor concentration of 0.1 – 1.0 mM, pH (6 to 9), reaction time (12 to 48 hours), and temperature (25 - 60 °C). We noted the production of a black color during the emergence of the nanoparticles throughout the reaction. This confirmed that iron oxide nanoparticles had formed. UV-Vis spectroscopy indicated that we had created iron oxide nanoparticles.......
Keywords: Box–Behnken Design, Dalbergia sissoo, green synthesis, Iron oxide nanoparticles, Response Surface Methodology, UV–Vis spectroscopy,
[1].
Ali, A., Zafar, H., Zia, M., Haq, I. U., Phull, A. R., Ali, J. S., & Hussain, A. (2016). Synthesis, characterization, applications, and challenges of iron oxide nanoparticles. Nanotechnology, Science and Applications, 9, 49–67. https://doi.org/10.2147/nsa.s99986
[2].
Ashrafi-Saiedlou, S., Rasouli-Sadaghiani, M., Fattahi, M., & Ghosta, Y. (2025). Biosynthesis and characterization of iron oxide nanoparticles fabricated using cell-free supernatant of Pseudomonas fluorescens for antibacterial, antifungal, antioxidant, and photocatalytic applications. Scientific Reports, 15(1). https://doi.org/10.1038/s41598-024-84974-0
[3].
Bhattacharya, M., Singh, A., & Ramrakhyani, C. (2014). Dalbergia sissoo – An important medical plant. Journal of Medicinal Plants Studies, 2(2), 76–82. http://www.plantsjournal.com
[4].
Bhattacharya, R., & Mukherjee, P. (2008). Biological properties of “naked” metal nanoparticles. Advanced Drug Delivery Reviews, 60(11), 1289–1306. https://doi.org/10.1016/j.addr.2008.03.013
[5].
Blaszczak-Boxe, A. (2017). Facts about iron. Live Science. https://www.livescience.com/29263-iron.html