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| Paper Type | : | Research Paper |
| Title | : | Electrophoretic Profile of Protein and Isozymes in Leaf Gall Of Pongamia Pinnata (L.) |
| Country | : | India |
| Authors | : | Richa Choudhary || Sanjay Kumar || Binay Kumar || De Ashwini Kumar |
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: | 10.9790/3008-1203060104  |
Abstract: In the present investigation the electrophoretic studies of protein and isozymes (peroxidase and polyphenol oxidase) in leaf galls of Pongamia pinnata induced by Aceria pongamiae were carried out. The results showed some variation in SDS-protein and isozymes activities banding patterns among leaf galls and normal leaf. Total twenty bands were observed in each lane of galled leaf sample, in comparison one less band was observed in normal leaf crude protein profiling of experimental plant. We observed that the intensity of protein bands was higher in galled leaf as compare to normal leaf protein. Intensity of polyphenol oxidase and peroxidase activity was found to be very high in galled leaves as compared to their normal counterparts.
Keywords: Isozymes, leaf gall, Pongamia pinnata, protein, SDS-PAGE.
[1] R.D. Yadav, S.K. Jain, S. Alok, S.K. Prajapati and A. Verma, Pongamia pinnata: An overview. IJPSR, 2(3), 2011, 494-500.
[2] S.V. Baste, Emission Characteristics of Pongamia pinnata (Karanja) Biodiesel and Its Blending up to 100% in a C.I. Engine, Research J. of Agri. and Forestry Sci., 1(7), 2013, 1-5.
[3] J. Sarnaik, A. Godbole, S. Andpunde, Integrating high conservation value native species into biofuel production for conservation and sustainable use of biodiversity, Applied Environmental Research Foundation (AERF), India, 2010.
[4] R.N. Coulson and J.A. Witter, Forest Entomology: Ecology and Management (John Wiley and Sons, New York, 1984) 669.
[5] J.D. Shorthouse and O. Rohfritsch, Biology of insect induced galls, Edited by J.D. Shorthouse, O. Rohfritsch (Oxford University Press, New York, Oxford, 1992) 285...
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| Paper Type | : | Research Paper |
| Title | : | Fly ash induced Morphological, Biochemical and Metal accumulation responses at different developmental stages in Glycine max (L.) Merrill. Revised |
| Country | : | India |
| Authors | : | Sheikh Ummar Bashir || Manik S. R || Wagay N. A || Mohammad Ayub Shah |
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: | 10.9790/3008-1203060513 |
Abstract: The research of many previous years has guaranteed the use of fly ash as a material for another green revolution. The present study was channeled to roll up the brilliance of the fly ash in agriculture meticulously. The experiments were conducted in pots of 20cm diameter with 6-7 kg capacity at Department of Botany SGBAU Amravati. The effect of varying levels of fly ash on the morphological and physiological response (protein content, carbohydrates, chlorophyll and carotenoids) of soya bean Glycine max (L.) Merrill. at three growth stages was observed. The varying levels of fly ash prepared with soil were 10%, 15%, 20%, 25%, 30% and a control of only garden soil was maintained. Experimental examination shows a momentous boost in plant biomass, yield and physiological response up to the level of 20 % fly ash from vegetative to pre flowering stage. After post flowering stage reduction in the physiological response was observed probably related to the distribution of nutrients at this stage.
Keywords: Agriculture, carbohydrates, Flyash, Glycine max, morphology, photosynthetic pigments proteins..
[1]. Jamwal N, (2003). Looks the ways to utilize fly ash. Down to earth, 12(3): 1-5.
[2]. Asokan P, Saxena M and Asolekar SR, (2005). "Coal Combustion Residues Environmental Implications and Recycling Potentials", Resources, Conservation & Recycling. 43: 239-252.
[3]. Sachdev RK, (2007). "Clean Coal Technologies – Indian Scenario", Third International Conference on Clean Coal Technologies for Our Future, Sardinia, Italy.
[4]. Arivazhagan K, Ravichandran M, Dube SK, Mathur VK, Ram KK, Yagnanarayana K, Kamal PMM, Sinha AK, Sarangi BD, Tripathi VKM, Gupta SK, Rajvir S, Mushtaq A, Thakur AS and Raghvendra N, (2011). Effect of the coal fly ash on agriculture crops: showcase project on use of fly ash in agriculture in and around thermal power station areas of national thermal power corporation Ltd., India. world of fly ash (WOCA) conference,in Denver,co,USA.
[5]. BN. Mittra, S. Karmakar, DK. Swain and BC. Ghosh, Fly ash a potential source of soil amendment and a component of integrated plant nutrient supply system. Fuel, 84 2005, 447-1451.
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| Paper Type | : | Research Paper |
| Title | : | Prognostic Value of p53 Expression in Intestinal Neoplasms - a Study in a Tertiary Health Care Centre |
| Country | : | India |
| Authors | : | Arputham Arunachalam || Babiya Infant Arockiasamy |
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: | 10.9790/3008-1203061420 |
Abstract: Colorectal cancer is one of the most common tumours of the world with the varying distribution. Though small intestine is an uncommon site for tumour, it accounts for significant number of gastro-intestinal malignancies. Pathological assessment of the resected specimen is mandatory for assessing the postoperative outcome and the rationale for adjuvant therapy. Among the various prognostic factors, Mutation of the p53 tumour suppressor gene, plays an important role in the progression of colorectal carcinoma and so it represents a clinically useful marker of prognosis. The present study evaluates the role of p53 in intestinal neoplasms by assessing its level of expression and to correlate the level of p53 expression with the stage of the colorectal adenocarcinomas so that its prognostic value in colorectal adenocarcinomas can be calculated
Keywords: Intestinal malignancies, colorectal adenocarcinoma, guardian genome, p53 expression, prognostic marker
[1] Norco masukura, Hicoko ohgaki, Rens Lambert. World cancer report. Lyon, IARC press, 2003;194-202.
[2] Desai PB. Carcinoma of the colon. Bombay hospital journal 2000;42:256-9.
[3] Hatzaras I, Palesty JA, Abir F et al. Small bowel tumours. Epidemiologic and clinical characteristics of 1,260 cases from the Connecticut tumour registry. Arch surg 2007;142:229.
[4] Crompton CC. Colorectal carcinoma Diagnostic, prognostic and molecular features. Mod Pathol 2003;16:376-88
[5] Chen Liu, Jame M. Grawford. The Gastrointestinal tract. In Robins and Cotran. Pathologic basis of disease, 8th edition. Philadelphia, Elsevier, 2010;822-25..
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| Paper Type | : | Research Paper |
| Title | : | Survey of Gastrointestinal Parasite of Cattle and Goat Slaughtered At Trans Amadi Abattoir, Port Harcourt-Rivers State |
| Country | : | Nigeria |
| Authors | : | Abah, A.E || Ebong, N.E. |
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: | 10.9790/3008-1203062124 |
Abstract: This work was to survey gastrointestinal parasites of cattle and goat slaughtered at Trans Amadi abattoir in Port-Harcourt. A total of 100 faecal samples of the ruminants were examined comprising of 50 cattle and 50 goats. Formol-ether concentration technique was used for sample analyses and examined with microscopically. The data obtained were subjected to simple statistical analysis using percentages to determine the prevalence rate in both ruminants studied. The result showed that 28(56.0%) of cattle and 23(46.0%) of the goat were infected with gastrointestinal parasites. The infection was slightly higher in the cattle than the goat though the difference was not statistically significant (P>0.05). Among the cattle, the organisms identified were Haemonchus contortus,Trichuris globulosa,Trichostrongylus columbriformis Monezia benedeni and Fasciola gigantica. The most prevalent organism was Haemonchus.................
Keywords: Gastrointestinal, parasite, Prevalence, Abattoir, Cattle, Goat. Port Harcourt .
[1] Anderson R.C (2000) Nematode Parasites of Vertebrates. Their Development and Transmission. 2ndedition. Wallingford, Oxon OX10 8DE, UK: CAB International; 2000.
[2] Ballweber L.R (2001). Veterinary Parasitology (Practical Veterinarian).(S.P.Messonniered.). Butterworth-Heinemann Publication. USA.
[3] Bariajaya, D; Estuningsh, E.S; Darmono, M.R; Knox, M.; Stoltz, D.R. and Wilson, A.J. (1995). The use of wormala in controlling gastrointestinal nematode infection in sheep under traditional grazing management.Indonesia Journal HmuTermakdanveterineer. 13: 49-55.
[4] Belem, A.M.G; Ouedraogo, O.P; and Bessin R.(2001). Gastrointestinal nematodes and cestodes of cattle in Burkina Faso. Biotechnology Agronomy Social Environment5(1), 17-21.
[5] Besier R.B and Love S.C.J (2003). Anthelmintic resistance in sheep nematodes in Australia: the need for new approaches. Australian Journal of Experimental Agriculture 43:1383-1391..
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| Paper Type | : | Research Paper |
| Title | : | Elemental Analysis of Dashmool Formulation by ICP-Ms Technique |
| Country | : | India |
| Authors | : | Saxena Abhishek || Chaudhary Yogita || Singh Manisha || Kumar |
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: | 10.9790/3008-1203062529 |
Abstract: Multi element analyses were carried out on Dashmool plants using ICP-MS Technique. Zn, Cu, Ni, Mn were screened. The elements present in medicinal plants play an important role in treatment of disease as discussed in Ayurvedas .On the basis of the different results of Dashmool may be more potent against the other disease. Dashmool plants showed the presence of essential elements that could enhance curative process of inflammation.
Keywords: Mass Spectrometer, Multi-Elemental Analysis, Dashmool plants
[1] Patra A,Mukhopadhyay AK & Ghosh A,Constitution of Aegle marmelos:Carbon-13 NMR spectra of aurapten and marmin,Indian Journal of Chemistry,Section B : Organic chemistry Including Medicinal Chemistry 17B(4) (1979) 385-7.
[2] Chatterjee A,Sen R & Ganguly D, On the constitution of the Active principles isolated from the matured bark of Aegle marmelos Correa ,Journal of American Chemical Society 71(1979) 606-609.
[3] 3.Chatterjee A & Chaudhary B,Occurrence of Auraptene,Umbelliferone,marmin,lupeol and skiammiainine in the root of aegle marmelos corre, Journal of the Indian chemical Society 37(1960) 334-336.
[4] Shoeb A,Randhir S & Satya P,Coumarins and Alkaloidof Aegle Marmelos, Phytochemical Reports 12 (1973) 2071-2072.
[5] Sharma B.Rattan RK & Sharma P, Constituents of leaves and fruits of Aegle marmelos, Section B: Organic Chemistry Including Medicinal Chemistry 19B (2) (1980) 162.
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| Paper Type | : | Research Paper |
| Title | : | Genetic Relationship amongSome Wheat Genotypes Using Ten ISSR Markers |
| Country | : | Iraq |
| Authors | : | Melathkadem FarhoodAL- Ghufaili || Attyaf Jameel Thamir Al-Tamimi |
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: | 10.9790/3008-1203063036 |
Abstract: Ten PCR DNA-based markers of ISSRs (inter- Simple Sequence Repeats)were used for revealing genetic relationship, genetic diversity and DNA fingerprint of ten wheat (Triticumaestivum L.) genotypes. Molecular size of amplified bands ranged (138-1717) bp in primer UBC852, this primer also gave higher number of main bands reached 19 band while primer 844B gave lower value for both main and amplified bands .Higher number of both amplified and monomrphic was 142 and 13 respectively in primer 17899A while primer HB12 gave only two monomorphic bands. Primers UBC811 and HB12 gave 11 polymorphic bands while primers 844A and 844B gave only two. Five unique bands in primers HB12 and HBS10 and one band in UBC881. Higher polymorphism produced by UBC811 while lower value by 17899A. The efficiency was higher in primer HB12 and lower...............
Keywords: Wheat, ISSR markers, Geneticdiversity, Dendrogram,.
[1]. Abbas, S.J.; Shah,S.R.U.; Rasool,G.andIqbal,A.(2008). Analysis of genetic diversity in Pakistani wheat varieties by using randomly amplified polymorphic DNA (RAPD) primers American-Eurasian Journal of Sustainable Agriculture, 2(1): 29-33.
[2]. Abou-Deif, M. H.; Rashed, M. A.; .Sallam, M. A. A.; Mostafa, E. A. H. and Ramadan,W.A.(2013).Characterization of Twenty Wheat Varieties by ISSR Markers. Middle-East Journal of Scientific Research 15 (2): 168-175.
[3]. Akkaya, M.S.; Bhagwat, A.A. and Cregan, P.B. (1992). Length polymorphisms of simple sequence repeat DNA in soybean. Genetics, 132: 1131–9.
[4]. Al–Badeiry , N. A.M.(2013).Molecular and Cytological Studies on Some Zea mays Varieties in Iraq .Phd thesis, University of Kufa ,Faculty of Science ,Department of Biology , Iraq.
[5]. Al-Judy, N.J. (2004).Detecting of DNA Fingerprints and Genetic Relationship Analysis in Local and Improved Rice (Oryza sativa L.)Varieties in Iraq Using RAPD Markers.Ph.D thesis, College of Science, Baghdad University, pp 166..
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| Paper Type | : | Research Paper |
| Title | : | Effect of Nano Silicon Dioxide Addition on Some Properties of Heat Vulcanized Maxillofacial Silicone Elastomer |
| Country | : | Iraq |
| Authors | : | Mustafa S. Tukmachi || Mohammed Moudhaffer M. Ali |
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: | 10.9790/3008-1203063743 |
Abstract: Silicone elastomer is widely used as the material of choice for fabricating maxillofacial prosthesis. However, silicone properties are far from ideal; low tear strength, low tensile strength and insufficient elasticity are the most undesirable properties. The purpose of this study was to evaluate the effect of addition of nano SiO2filler on tear strength, tensile strength, elongation at break, hardness and color of Cosmesil M-511 HTV maxillofacial silicone elastomer. Nano SiO2was added to the silicone base in concentrations of 4%, 5% and 6% by weight. Silicone with 0% nano filler served as a control. Tear test was done according to ISO 34-1. Tensile and elongation test was done according to ISO 37. Shore A hardness test was done according to ISO 7619. Visual color measurement test was done according to ASTM D1535................
Keywords: HTVmaxillofacial silicone, nano SiO2, tear strength, tensile strength, Shore A hardness
[1] U. Maller, K. Karthik and S. Maller. Maxillofacial Prosthetic Materials - Past and Present Trends,J Ind Aca Dent Spec 1(2), 2010, 42-44.
[2] C. Andres, S. Haug, C. Munoz and G. Bernal. Effects of environmental factors on maxillofacial elastomers: Part I--Literature review, J Prosthet Dent 68(2), 1992, 327-330.
[3] G. Barnhart. A new material and technic in the art of somato-prosthesis,J Dent Res 39, 1960, 836-844.
[4] C. Andres, S. Haug, D. Brown and G. Bernal. Effects of environmental factors on maxillofacial elastomers: Part II--Report of survey,J Prosthet Dent 68(3), 1992, 519-522.
[5] T. Aziz, M. Waters and R. Jagger. Analysis of the properties of silicone rubber maxillofacial prosthetic materials, J Dent 31(1), 2003, 67-74..
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| Paper Type | : | Research Paper |
| Title | : | Anti-Diabetic and Antihyperlipidemic Activities of Alternanthera Sessilis in Experimentally Induced Type 2 Diabetes |
| Country | : | India |
| Authors | : | Deepthi Rayilla || P Goverdhan |
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: | 10.9790/3008-1203064447 |
Abstract: The antidiabetic and antihyperlipidemic effects of ethanolic extract of whole plant of Alternanthera sessilis (EEAS) was investigated in Streptozotocin (STZ) induced type 2 diabetic rats. Diabetes was confirmed after 3 days of single intraperitoneal injection of STZ (35mg/kg) in albino Wistar rats. EEAS (100 and 200mg/kg) and glibenclamide (10mg/kg) orally administered daily for 15 days respectively, blood was withdrawn for glucose determination on 1, 7, 15 days respectively. On 15th day, overnight fasted rats were sacrificed and blood was collected for determination of high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), total cholesterol (TC), triglycerides (TG). EEAS at doses of 100mg/kg and 200mg/kg showed significant reduction in blood glucose and lipid profiles when compared to diabetic control group. We concluded that EEAS possess antidiabetic and antihyperlipidemic activities.
Keywords: Alternanthera sessilis, Streptozotocin, Antidiabetic, Antihyperlipidemic
[1] Holman, R.R., Turner , R.C. Oral agents and insulin in treatment of NIDDM. Pickup, J., Williams, G (Ed), Text book of diabetes, Blackwell, oxford1991, pp.467-479.
[2] B.S. Ashok Kumar, K. Lakshman, K.N. Jayaveea. Antidiabetic, antihyperlipidemic activities of methanolic extract of Amaranthus viridis Linn. in alloxan induced diabetic rats. Experimental & toxicologic pathology 2010, 1-5.
[3] Chhanda Mallick, Raj Kumar Maiti, Debidas Ghosh. Comparative study on Antihyperglycemic and Antihyperlipidemic effects of separate and composite extracts of seeds of Eugenia jambolana and roots of Musa paradisiaca in Streptozotocin induced diabetic male albino rats. Iranian journal of Pharmacology & Therapeutics 2006. 5, 27-33.
[4] Sumana, G., Suryawamshi S.A. Effect of vinca rosea extracts in treatment of streptozotocin diabetes in male albino rats. Indian journal of experimental biology 2001.
[5] Burke J.P., Williams, K.M.V. et al. A population perspective on diabetes prevention:whom should we target for preventing weight? Diabetes Care26, 1999-2004.
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| Paper Type | : | Research Paper |
| Title | : | Anti-Cancer Therapy: Chlorogenic Acid, Gallic Acid and Ellagic Acid in Synergism |
| Country | : | India |
| Authors | : | Archana Moon || Trupti Agrawal || Pooja Gupta || Nikita Kondlekar || Amit Taksande |
|
: | 10.9790/3008-1203064852 |
Abstract: Phytochemicals have been used as effective agents for treating illnesses. Its use has been widely increased in the present scenario where commercial drugs are carrying a huge burden of side effects. Many phytochemicals have potent anticancer properties. The present study was undertaken to study the synergistic effect of anticancer phytochemicals Chlorogenic acid, Gallic acid and Ellagic acid on MDA MB 231 Breast cancer cells. The study was evaluated using cytotoxic assay such as MTT assay and migration assay via scratch well assay. The results demonstrate that the selected phytochemicals are highly cytotoxic to the cells and thus, synergistic studies were performed and the most cytotoxic combinations were proceeded with further assay which reveals that there is no migration observed in the presence of selected phytochemical combinations, thus revealing that treatment must be anti-metastatic
Keywords: Chlorogenic acid, Ellagic acid, Gallic acid, MDA MB 231, MTT Assay, Scratch Assay
[1]. T.Y. Kang, H.R. Yang, J. Zhang, D. Li, J. Lin, L. Wang and X. Xu, The studies of Chlorogenic Acid Antitumour Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression. Journal of Analytical Methods in Chemistry, Volume 2013, Article ID 617243, 2013. [2]. G. Noratto, W. Porter, D. Byrne and L. Cisneros-Zevallos, Identifying Peach and Plum Polyphenols with Chemopreventive Potential against Estrogen-Independent Breast Cancer Cells, Journal of Agricultural and Food Chemistry, Vol.57 (12), 2009, pg. 5219-26.
[3]. R. Xu, Q. Kang, J. Ren, Zukun, Z. Li and X. Xu, Antitumor Molecular mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and inhibiting Gene β-Catenin. Journal of Analytical Methods in Chemistry, Vol. 2013, 2013, Article ID 951319.
[4]. S. Shahrzad, K. Aoyagi, A. Winter, A. Koyama, and I. Bitsch, (December 2000) Pharmacokinetics of Gallic Acid and Its Relative Bioavailability from Tea in Healthy Humans. Human Nutrition and Metabolism–Research Communication, 19, 2000.
[5]. A. Faried, D. Kurnia, L.S. Faried, N. Usman, T. Miyazaki, H. Kato and H. Kuwano, Anticancer effects of gallic acid isolated from Indonesian herbal medicine, Phaleriamacrocarpa (Scheff.) Boerl, on human cancer cell lines. International Journal of Oncology, Vol 30, 2007, pg. 605-613.
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| Paper Type | : | Research Paper |
| Title | : | Indigenous Claim Supports in vitro Antioxidant and Cytotoxic Screening of certain South Indian Medicinal Plants |
| Country | : | India |
| Authors | : | ShakkeelaYusuf || K.K.Srinivasan || Jyoti Harindran |
|
: | 10.9790/3008-1203065356 |
Abstract: Plant drugs enjoy much acclaim and wide acceptability even in the midst of amazing advancements in modern medicine. Current research in drug discovery from medicinal plants involves a multifaceted approach combining botanical, phytochemical, biological and molecular techniques. Evaluation of certain selected south Indian medicinal plants popularly used in Indian system of medicine has been taken up for their antioxidant and cytotoxic activities. The plants selected were Heliotropium indicum, Schleichera oleosa, Shorea robusta, Symplocos cochinchinensis and Wrightia tinctoria. Antioxidant activities involved DPPH, total antioxidant, iron chelating and nitric oxide assays. Cytotoxicity assay was carried out by MTT using SKMEL-28 and HCT-15 cell lines. Results obtained could very well support the therapeutic claims made for the investigated plants.
Keywords: antioxidant, cytotoxic, DPPH, iron chelating, MTT
[1]. Srinivas K, Rao MEB, Rao SS. Anti-inflammatory activity of Heliotropium indicum linn.and leucas aspera spreng. in albino rats. Indian J Pharmacol. 2000;32:37–8.
[2]. Bhatia H, Kaur J, Nandi S, Gurnani V. A review on Schleichera oleosa : Pharmacological and environmental aspects. JOPR J Pharm Res [Internet]. Elsevier Ltd; 2012;6(1):224–9. Available from: http://dx.doi.org/10.1016/j.jopr.2012.11.003
[3]. Yusuf, Shakkeela KKS. GC-MS Analysis of Oil Isolated and Antioxidant Activity of Shorea Robusta Oleoresin. Am J Pharm Heal Res. 2015;3(11):1–8.
[4]. Devi SL, Divakar MC. Wrightia tinctoria ( Roxb ) R . Br . - An updated Review. Hygeia JD MED. 2014;6(April):95–105.
[5]. C.P.Khare. Indian Medicinal Plants
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| Paper Type | : | Research Paper |
| Title | : | Association between Elevated Serum Ferritin and HBA1C in Type 2 Diabetes Mellitus |
| Country | : | India |
| Authors | : | Priyanka Vaitla || MD Sabiullah || N.Vani |
|
: | 10.9790/3008-1203065759 |
Abstract:Background and objectives : Diabetes is a disease of metabolic dysregulation. Recent studies have emphasized the role of serum ferritin in insulin resistance and the incidence of diabetes mellitus. However, the role of ferritin as a marker of iron overload in pancreatic damage and peripheral insulin resistance or its role as an inflammatory marker is not clear. The aim of this study is to establish an association between serum ferritin, FBS and HbA1c in type 2 diabetes mellitus and to evaluate the role of serum ferritin on glycemic status. Methods : A case control study was done with 90 type 2 DM patients. FBS,PLBS, Hb%, HbA1c% and serum ferritin were measured.......
Keywords: Diabetes mellitus, ferritin, oxidative stress, HbA1c
[1] Nitin S. HbA1c and factors other than diabetes mellitus affecting it. Singapore Med Journal 2010; 51: 616-622.
[2] Jiang R, Manson JE, Meigs JB, Ma J, Rifai N, et al. (2004) Body iron stores in relation to risk of type 2 diabetes in apparently
healthy women. JAMA 291: 711–717.
[3] Radoi V, Lixandru D, Mohara M, Virgolici B. Advanced glycation and products in diabetes melltus. Mechanism of action and
focused treatment. Proc.Rom.Acad. Series B,2012; 1:9-19.
[4] Herbert V. Everyone should be tested for iron disorders. J Am Diet Assoc 1992;92:1502-1509.76
[5] Swaminathan S, Fonseca VA, Alam MG, Shah SV. The role of iron in diabetes and its complications. Diabetes care.
2007;30(70:1926-1933)
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| Paper Type | : | Research Paper |
| Title | : | Simultaneous Estimation of Hydrochlorothiazide and Losartan Potassium in Bulk Solid Dosage Form by Chemometric Assisted Spectrophotometric Methods |
| Country | : | India |
| Authors | : | Maneesha.Narisetty || A. Elphine Prabahar || P.V.Suresh || RamaRao.N |
|
: | 10.9790/3008-1203066065 |
Abstract: A simple UV-visible spectroscopic method was developed and Chemo metric designs were applied for the simultaneous estimation of hydrochlorothiazide (HCT) and losartan potassium (LST) in bulk and solid dosage form. The spectroscopic method was developed by using alcohol as solvent for the two drugs and the data generated from the spectra were mined by using Chemo metric methods such as bi-linear regression analysis, Cramer's matrix method, Method of least squares, The wavelengths selected for all the above methods were 271 nm (wavelength of maximum absorption; λmax of HCT), 213 nm (wavelength of maximum absorption; λmax of LST)..............
Keywords: uv-visible spectroscopy, chemometrics, hydrochlorothiazide and losartan potassium
[1]. Matero S (2010) Chemometrics Methods in Pharmaceutical Tablet Development and Manufacturing Unit Operations. Publications of the University of Eastern Finland Dissertations in Health Sciences.
[2]. Mocak J (2012)Chemometrics in medicine and pharmacy. Nova Biotechnologicaet Chimica.
[3]. D.L.Massaret al., acahemometric in pharmaceutical analysis.
[4]. V.Aparna, D.Sireesha, Dr.Vasudhabakshi UV- Spectrophotometry pharma research library.
[5]. A.Sankar, ThanagarasuVetrichelvan ,Devashya Venkappaya UV-Speactrophotometric Degruyter, sep 2011.
[6]. Harshad O. Kaila UPLC Method Saurashta University,2011
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| Paper Type | : | Research Paper |
| Title | : | Knowledge, Attitudes and Practice of Undergraduate Students in University of Nigeria Enugu campus (UNEC) towards Hepatitis B |
| Country | : | Nigeria |
| Authors | : | Kosisochi Chinwendu Amorha || Martin Ifeanyi Chiebue || Ebere Emilia Ayogu || Onyinye Blessing Ukoha-Kalu || Mathew Jegbefume Okonta |
|
: | 10.9790/3008-1203066671 |
Abstract: Undergraduate students represent a population that is at high-risk for acquiring and spreading hepatitis B infection (HBV). This study was designed to evaluate the knowledge, attitudes and practice of undergraduate students in the University of Nigeria Enugu Campus (UNEC) towards Hepatitis B. During April to May 2014, a cross-sectional and descriptive survey was conducted amongst undergraduate students of UNEC. A 33-item structured questionnaire on statements concerning knowledge base of HBV, attitudes and practices towards hepatitis B were distributed to 360 students. Statistical analysis was carried out with the Statistical Package for Social Sciences (SPSS) v 16.0 using the independent sample's t-test and ANOVA test with level of significance set at P ≤ 0.05. Majority had heard of hepatitis (93.0%) and hepatitis B (89.9%). Less than half (35.9%) thought they could get hepatitis B infection. Only 25.6% had gone for a hepatitis B screening and just 33.0% had been vaccinated against HBV.............
Keywords: Attitudes, Hepatitis B, Knowledge, Practice, Undergraduate Students
[1]. World Health Organization (WHO). Hepatitis B [monograph on the internet]. 2015. [cited 2016 Mar 29]. Available from: http://www.who.int/immunization/diseases/hepatitisB/en/.
[2]. World Health Organization (WHO). Hepatitis B. Fact sheet [monograph on the internet]. 2015. [cited 2016 Mar 30]. Available from: http://www.who.int/mediacentre/factsheets/fs204/en/.
[3]. J.F. Perz, G.L. Armstrong, L.A. Farrington, Y.J. Hutin, and B.P. Bell. The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide, Journal of Hepatology, 45, 2006, 529–538.
[4]. B. Musa, S. Bussell, M.M. Borodo, A.A. Samaila and O.L. Femi. Prevalence of hepatitis B virus infection in Nigeria, 2000-2013: A systematic review and meta-analysis, Nigerian Journal of Clinical Practice, 18, 2015, 163-172.
[5]. I. Isa, M. Aminu, S.A. Abdullahi, M.A. Sani, and D.E. Akafyi. Seroprevalence of hepatitis B virus in a tertiary institution in North Western Nigeria, African Journal of Microbiology Research, 9, 2015, 171-179.
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| Paper Type | : | Research Paper |
| Title | : | Antimicrobial Activity of Seed Extracts from Albizia bernieri E. Fourn. (Fabaceae) |
| Country | : | East Africa |
| Authors | : | Lovarintsoa Judicael Randriamampianina || Vahinalahaja Eliane Razafintsalama || Danielle Aurore Doll Rakoto || Hanitra Ranjana Randrianarivo || Victor Louis Jeannoda |
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: | 10.9790/3008-1203067279 |
Abstract: This work aimed at assessing the antimicrobial activity of Albizia bernieri seed extracts. Methanol extract (SME), alkaloids extracted under basic (Alk1) and acidic (Alk2) conditions, polar saponosides (Sap1) and less polar saponosides (Sap2) obtained by n-butanol fractionation were used. Their activities were tested against 9 pathogenic germs including 4 Gram (+) bacteria, 5 Gram (-) bacteria and 1 yeast using disc diffusion and microdilution methods. Regardless the method used to assess the antimicrobial activity, all extracts were efficient but their effects depended upon the germs tested. They all displayed a broad spectrum activity. Alkaloids were by far the most efficient with an excellent effect (Minimum Inhibitory Concentration or MIC<100μg/ml) against all germs tested............
Keywords:Albizia bernieri, antimicrobial activity, disc diffusion method, microdilution method, minimum bactericidal concentration, minimum inhibitory concentration.
[1]. C. Zongo, A. Savadogo, M.K. Somda, J. Koudou and A.S. Traore, In vitro evaluation of the antimicrobial and antioxidant properties of extracts from whole plant of Alternanthera pungens H.B. & K. and leaves of Combretum sericeum G. Don. International Journal of Phytomedicine, 3, 2011, 182-191.
[2]. J.B. Dalmarco, E.M. Dalmarco, J. Koelzer, M.G. Pizzolatti and T.S. Fröde. Isolation and identification of bioactive compounds responsible for the anti-bacterial efficacy of Lotus corniculatus var. São Gabriel, International Journal of Green Pharmacy, 4, 2010, 108-114.
[3]. O. Stefanovic and L. Comic, Inhibitory effect of Cytisus nigricans L. and Cytisus capitatus Scop. on growth of bacteria, African Journal of Microbiology Research, 5(27), 2011, 4725-4730.
[4]. O. Odeyemi, A.O. Oluduro and O.M. David, Evaluation of Antimicrobial Activities of Albizia zygia DC Leaf Extracts against Some Clinically Important Pathogens, Journal of Natural Sciences Research, 4(19), 2014, 98-105.
[5]. P.V. Rajalakhmi and K. Senthil, Flavonoid content and antibacterial activity of Albizia julibrissin Durazz. Leaf, stem and flower extracts against clinically isolated bacterial pathogens, International Journal of Pharmaceutical Sciences, 6(11,) 2014, 506-508
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| Paper Type | : | Research Paper |
| Title | : | Nanoscale Biomaterials for 3D printing |
| Country | : | India |
| Authors | : | Akshay Bhatt || Anand Anbarasu |
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: | 10.9790/3008-1203068086 |
Abstract: Bio printing is an emerging tool for differentiating stem cells in 3D patterns. This technique provides the platform for regeneration of organs and tissues in a systematic manner.The use of suitable biomaterial with its crosslinking property on addition of a cross linker gives the exact structure with which we can grow stem cells into a specific tissue or organ. There exists a number of conventional biomaterials used as bioinks for 3D bioprinting and other biomedical applications, but due to its macro sized structure the applicability had been restricted to certain areas. In such cases nano-biomaterials play a key role in enhancing the physicochemical properties of existing biomaterials. Literature survey shows a wide range of conventional hydrogels available for printing and one can select these biomaterials according to the tissue to be regenerated. For example, regeneration of bone or cartilage requires relatively strong material and in such case soft materials will be inappropriate for the cells to differentiate into bone/cartilage tissue.........
Keywords: 3D Bioprinting, bio-inks, biomaterials, nanomaterials, tissue engineering and regenerative medicine
[1]. Armando Salim Munoz-Abraham & Manuel I. Rodriguez-Davalos, A.B.& B.W.& J.P.G.& D.C. Mulligan, 3D Printing of Organs for Transplantation: Where AreWe and Where Are We Heading?, Tissue Eng. Regen. curr trans (2016) 1–7.
[2]. B. Aleksander Skardal, J. Zhang, L. McCoard, S. Oottamasathien, G.D. Prestwich, G.D. Prestwich, A. Skardal, J. Zhang, L. McCoard, S. Oottamasathien, Dynamically Crosslinked Gold Nanoparticle – Hyaluronan Hydrogels, Adv. Mater. 22 (2010) 4736–4740. doi:10.1002/adma.201001436.
[3]. J. Mark, C. Jiang, M. Tang, Simultaneous curing and filling of elastomers, Macromolecules. 17 (1984) 2613–2616. doi:10.1021/ma00142a026.
[4]. H. Zou, S. Wu, J. Shen, Polymer/silica nanocomposites: preparation, characterization, properties, and applications., Chem. Rev. 108 (2008) 3893–3957. doi:10.1021/cr068035q.
[5]. I.Y. Jeon, J.B. Baek, Nanocomposites derived from polymers and inorganic nanoparticles, Materials (Basel). 3 (2010) 3654–3674. doi:10.3390/ma3063654.
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| Paper Type | : | Research Paper |
| Title | : | Isolation and Purification of Microbial Glucan sucrase from Decayed Tooth extracts |
| Country | : | India |
| Authors | : | Polampalli Phani Deepika || Dr. D.Sudheer kumar || Prof M.Lakshmi Narasu || Dr.P.Srinivas |
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: | 10.9790/3008-1203068791 |
Abstract: The present objective of the research work is isolation of glucan sucrase from the decayed tooth extracts by carrying out inoculation of dental samples into Todd-Hewitt broth and centrifugation .The culture supernatant is subjected to protein fractionation and supernatant collected is purified for the enzyme by anion exchange chromatography and gel permeation. The solution from the above purification procedures are finally subjected to electrophoresis using SDS-PAGE. Molecular weight bands are compared and the glucan sucrase analysed from purified band
Keywords:Tooth decay extracts, Fractionation, Gel permeation, Ion exchange chromatography, PAGE electrophoresis
[1]. Banas, J.A. & Vickermann, M.M. 2003, "Glucan-binding proteins of the oral streptococci", Critical reviews in oral biology and medicine : an official Publication of the American Association of Oral Biologists, vol. 14, no. 2, pp. 89-99.
[2]. Chassy B.M 1976 A gentle method for the lysis of oral Streptococci. Biochem Biophys Res commun 69; 603-608.
[3]. Hendrick Hellmuth, Sabine wittrock, slavko kralj. Engineering the glucan sucrase GTFR enzyme reaction amd glycosidic bond specificity toward tailor made polymer and oligosaccharide products. Biochemistry 2008, 47, 6678-6684.
[4]. Keevil.C, A. West, P.Marsh, and D.C Ellwood. 1983 Batch versus continuous culture studies of glucosyl transferase synthesis in oral streptococci. P. 189-200.
[5]. Lehninger, A.L., Nelson, D.L. and Cox, M.M (2004) Lehninger Principles of biochemistry, (4th edition); W.H. Freeman&co; Newyork, USA. 191-225.
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| Paper Type | : | Research Paper |
| Title | : | Change in Alkaline Phosphatase Activity Induced By Tetracycline in Freshwater Mussel, Parreysia cylindrica (Annandale and Prashad |
| Country | : | India |
| Authors | : | H. P. Nandurkar |
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: | 10.9790/3008-1203069294 |
Abstract: Alkaline phosphatase is a non-specific hydrolase enzyme. This enzyme is responsible for transphosphorylation and has an important role in the general energetics of an organism. Internal milieu of the organ can be assessed by alteration in the enzymatic activity as it is a tool to indicate the stress offered by various chemical compounds. In the present study, Alkaline phosphatase activity is measured in a fresh water mussel Parreysia cylindrica after acute and chronic exposure to tetracycline, one of the broad spectrum antibiotics. P. cylindrica are exposed to LC50/2 (166.54PPM) for acute treatment upto 96 hrs and LC50/10 (33.30 PPM) for chronic treatment upto 21 days in gills, mantle and digestive gland. In tetracycline exposed bivalves, alkaline phosphatase activity was greatly increased in gills (+26.37%).............
Keywords: Alkaline phosphatase activity, P. cylindrica, tetracycline
[1]. Ranilalitha P., Sukumaran1 M. and Raveendran S., (2014) Effect of TBTCL on phosphatases activity in estuarine edible clam, Anadara rhombea Born (Bivalvia: Mollusca) International Journal of Pure and Applied Zoology, Vol.2(4): 315-320.
[2]. Joshi, V.M. and Desai, A.K., (1981) Effect of sublethal concentration of monocrotophos on acid and alkaline phosphatases activities in the tissues of freshwater fish, Tilapia mossambica. J. Anim. Morphol. Physiol. 28: 221-228.
[3]. Surendran, S. (1994) Levels of Alkaline and acid phosphatases in the brain of fish. Ecotoxicol. Environ. Monit. 4(1): 71-73.
[4]. Sonatakke, and Sunita Jadhav, (1997) Changes in the acid and alkaline phosphabase activity in the tissues of Thiara tuberculata after exposure to heavy metals, Ind. J. Environ. Toxicol., 7(1): 32-35.
[5]. Hansen, J.I., Mustafa, T. and Depledge, M.,(1992) Mechanism of copper toxicity in the shore crab, Carcinus moenas. Mar. Biol.,114(2): 253-257.