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| Paper Type | : | Research Paper |
| Title | : | Amylase Activity of a Starch Degrading Bacteria Isolated From Soil Around Tulja-Bhavani Temple of Tuljapur. |
| Country | : | India |
| Authors | : | Tole S.B. || Fawade M .M. || Patil S.M. |
Abstract: The amylase can be derived from several sources such as plants, animals and microbes. The microbial amylases meet industrial demands because it is economical when produced in large quantities. The aim of current study was to isolate amylase producing bacteria from the soil samples collected from Tulja Bhavani Temple of Tuljapur. The isolation was done by serial dilution and plating method. Total 20 bacterial isolate were isolated from the collected soil samples. All isolates were screened for amylolytic activity by starch agar plate method. Among 09 bacterial isolates, only 03 isolates showed the amylolytic activity. Out of there one isolate was selected on the basis of maximum hydrolysis for further study. The 16 s r RNA sequences of selected strain was isolated and sequenced.
Keywords: Amylase production, Enzyme purification, Soil bacteria, Sephadex, SDS-PAGE.
[1]. Pandey, A., Nigam, P., Soccol, C. R. V. T., Soccol, V., Singh, D., & Mohan, R."Advances in microbial amylases". Biotechnology and Applied Biochemistry, 31, 135-152, (2000). Journal of Applied & Environmental Microbiology 174
[2]. Gupta, R., Gigras, P., Mohapatra, H., Goswami, V. K., & Chauhan, B. "Microbial α-amylases: a biotechnological perspective", Process Biochemistry, 38 (11), 1599-1616, (2003).
[3]. Drauz, K., Gröger, H., & May, O. (Eds.) Enzyme catalysis in organic synthesis: a comprehensive Handbook, John Wiley & Sons, (2012), Souza, P. M. D., "Application of microbial α-amylase in industry-A review", Brazilian journal of microbiology, 41 (4), 850-861, May (2010).
[4]. Das, S., Singh, S., Sharma, V., & Soni, M. L. "biotechnological applications of industrially important amylase enzyme". International Journal of Pharma & Bio Sciences, 2 (1), March (2011).
[5]. Oboh, G. "Isolation and characterization of amylase from fermented cassava (Manihot esculenta Crantz) wastewater". African journal of biotechnology, 4 (10).October (2005).
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| Paper Type | : | Research Paper |
| Title | : | Morphometric Analysis of Giant Honeybee, Apis Ddorsata Worker Bees of Different Areas of Mysore District, Karnataka, India |
| Country | : | India |
| Authors | : | Bidisha Rajak || S. Basavarajappa |
Abstract: The morphological characters of A.dorsata worker bees were analyzed after collecting moribund worker bees from different areas in Mysore District that experiences climate of semi-malnad type in southern dry zone part of Karnataka. Around 32 morphological characters were measured with the help of computer aided microscope Axion Vision ref. 4.8 software. The thorax, abdomen and overall body length showed significant variation between the workers bees. However, head length didn't show significant variation (F=1.058; P>0.05) between the worker bees which belong to different areas. Interestingly, the head breadth, tongue length, mandible and antenna length showed significant variation (F= 6.599; P>0.05) between the worker bees collected from different areas of Mysore District
Keywords: Apis dorsata, worker bees, morphometric traits, Mysore District. * Corresponding Author.
[1]. Abou-Shaara, H. F. and Al-Ghamdi. 2012. Studies on wings symmetry and honey bee races discrimination by using standard and geometric morphometrics. Biotechnol. Animal Husbandry. 28: 575-584.
[2]. Adl, M. B. F., Gencer, H. V., Firati, C. and R, Bahreini. 2007. Morphometric characterization of Iranian (Apis mellifera meda), Central Anatolian (Apis mellifera anatoliaca) and Caucasian (Apis mellifera caucasica) honeybee populations. J. Apic. Res. 46: 225-231.
[3]. Alpatov, W. W. 1929. Biometrical studies on variation and races of the honey bee (Apis mellifera L). The Quarterly Review of Biol. 4: 1-58.
[4]. Amssalu, B., Nuru, A., Radloff, S. E. and H. R. Hepburn. 2004. Multivariate morphometric analysis of honeybees (Apis mellifera) in Ethiopian region. Apidologie 35: 71-81.
[5]. Andere, C., C. Garcı´a, Marinelli, C., Cepeda, R., Rodrı´quez, E. M. and A. Palacio. 2008. Morphometric variables of honeybees Apis mellifera used in ecotypes characterization in Argentina. Ecol. Model. 214: 53-58.
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| Paper Type | : | Research Paper |
| Title | : | Abortifacient Potential Effect of Aqueous Extract of Millittiaaboensis on Reproductive Health of Matured Wistar Rats |
| Country | : | Nigeria. |
| Authors | : | B. M. Onyegeme-Okerenta || F. C.Anacletus, K. |
Abstract: The present study evaluated the abortifacient potential of aqueous extract of Millittiaaboensis on reproductive health of matured Wistar rats by assessing some fertility indices, hormonal profile and litter size of pregnant wistar rats. A total of thirty (30) matured female wistar ratswere used for this study. Each matured female rat was mated with two male rats (2:1) until pregnancy was established using vaginal smear method and cage side observations. The pregnant rats were randomized into six (6) groups with five (5) animals in each group. Group 1 animals served as control group and were not treated with the extract but provided with food and water ad libitum. Groups 2,3,4,5 and 6 received concentrations of 1000,2000,3000,4000 and 5000mg/kg body weight of the extract accordingly
Keywords: Abortification, Millittiaaboensis, Fertility Indices, Reproductive Health
[1]. M.T. Yakubu, A.T. Oladiji and M.A. Akanji, Evaluation of biochemical indices of male reproductive function and testicular histology in wistar rats following chronic administration of aqueous extract of Fadogiaagrestis (Schweinf. Ex Heirn) stem, African Journal of Biochemistry Research, 1, 2007, 156–163.
[2]. M.T. Yakubu, M.A. Akanji and A.T. Oladiji, Evaluation of anti-androgenic potentials of aqueous extract of Chromolaenaodoratum (L.) K. R. leaves in male rats, Andrologia, 39, 2007, 235–243.
[3]. H.E. Ritchie, The safety of herbal medicine use during pregnancy, Frontiers in Fetal Health, 3, 2001, 259- 266.
[4]. F. Brinker, Inhibition of endocrine function by botanical agents, Anti-gonadotropic activity, British Journal of Phytotherapy, 4, 1997, 123–145. [5]. S.K. Gark, V.S. Mathur and R.R. Chaundhury, Screening of Indian plants for antifertility activity, Indian Journal of Experimental Biology, 16, 1978, 107–18..
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| Paper Type | : | Research Paper |
| Title | : | Identification and Characterization of Some Species of Cyanobacteria, Chlorophyta and BacillariophytaUsing Fourier-Transform Infrared (FTIR) Spectroscopy |
| Country | : | India |
| Authors | : | T. Özer || D. Yalçın || I. Açıkgöz Erkaya || A. U. Udoh |
Abstract: In this study FTIR was used to determine and identify 12 species of freshwater microalgae. Cyanobacteria, Chlorophyta and Bacillarophyta species were cultured in Allen media in batch culture. These algae were collected from different freshwaters in Ankara, Turkey. FTIR spectroscopy is easy to use and a robust technique allowed for macromolecular analysis and discriminates different genius of microalgae. FTIR enables discrimination at the strain level. When compared with identified molecular fingerprints regions (1500-1200 cm-1) in previous studies, our best separation range of spectral bands (1660-1190 cm-1) were similar. The results show that the FTIR technique has the potential to become applicable in the discrimination of Cyanobacteria, Chlorophyta and Bacillarophyta species.
Keywords: Characterization, FTIR, Microalgae, Spectroscopy
[1]. Murdock J.N. and Wetzel D.L., FT-IR Microspectroscopy Enhances Biological and Ecological Analysis of Algae, Applied Spectroscopy Reviews, 44, 2009, 335- 361.
[2]. Kansiz M., Heraud P., Wood B., Burden F., Beardall J. and McNaughton D, Fourier transform infrared microspectroscopy and chemometrics as a tool for the discrimination of cyanobacterial strains, Phytochemistry, 52, 1999, 407–417.
[3]. Beardall J., Berman T., Heraud P., Kadiri M.O., Light B.R., Patterson G., Roberts S., Sulzberger B., Sahan E., Uehlinger U. and Wood B, A comparison of methods for detection of phosphate limitation in microalgae, Aquat. Sci. , 63, 200,107-121.
[4]. Benning L.G., Phoenix V.R., Yee N., and Tobin M.J., Molecular characterization of cyanobacterial silicification using synchrotron infrared micro-spectroscopy. Geochim. Cosmochim. Acta, 68, 2004, 729-741.
[5]. Benning L.G., Phoenix V.R., Yee, N. and Konhauser K.O., The dynamics of cyanobacterial silicification: An infrared micro-spectroscopic investigation, Geochim. Cosmochim. Acta, 68, 2004, 743-757
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| Paper Type | : | Research Paper |
| Title | : | Evaluation of Anti-Depressentactivity of Ethanol extract of DrypetusRoxburgii |
| Country | : | India |
| Authors | : | Akshara || Krupanidhi A.M || Ashok R .Chanchi || Akshaykumar K || Yashaswini.T |
Abstract: Depression is the leading cause of disability and the fourth leading contributor to the global burden of disease in 2000. Today, depression is already the secondcause in the age category 15-44 years for both sexes combined. The present study was designed to evaluate the anti‐depressant activity of ethanol extract of the leaves of Drypetusroxburgii belonging to family Euphorbiaceae by using actophotometer and forced swimming test (FST) in Swiss albino mice..........
Keywords: Drypetusroxburgii, Forced swim test, anti‐depressant, actophotometer
[1]. WHO. The World Health Report. Mental health: New understanding new hope. WHO, Geneva 2001.
[2]. Reynolds EH. Brain and mind: a challenge for WHO. Lancet 2003;361:1924–25
[3]. Cheung IW, Nakayama S, Hsu MN, Samaranayaka AG, Li-Chan EC. Angiotensin-I converting enzyme inhibitory activity of hydrolysates from oat (Avenasativa) proteins by in silico and in vitro analyses. J Agric Food Chem 2009; 57(19):9234-42.
[4]. Ryan D, Kendall M, Robards K. Bioactivity of oats as it relates to cardiovascular disease. Nutr Res Rev 2007; 20(2):147-62.
[5]. Galdino PM, Nascimento MV, Sampaio BL, Ferreira RN, Paula JR, Costa EA. Antidepressant-like effect of LafoensiapacariA. St.-Hil. Ethanolic extract and fractions in mice. J Ethnopharmacol 2009; 124(3):581-5.
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| Paper Type | : | Research Paper |
| Title | : | Antimicrobial Effect of SimaroubaGlauca( LakshmiTaru) on Opportunistic Pathogens in HIV/AIDS patients |
| Country | : | India |
| Authors | : | JancyVarghese || S.Rajamani || Betty Daniel |
Abstract: Severalplant extracts exhibit antimicrobial activity against microbial pathogens. This suggests that plants which manifest relatively high levels of antimicrobial action may be the source of compounds that can be used to inhibit the growth of human pathogen[4] One of the ethnomedical plants Simaroubaglauca was investigated for Gas Chromatography Mass Spectrometry ( GCMS ) analysis to determine the chemical constituents present in different extracts of its leaves. The powdered leaf was subjected to successive extraction with five organic solvents. Out of 88 compounds reported most of the compounds were found to be medicinally effective, which could be useful against opportunistic infections in patients with poor immunity..........
Keywords:Simaroubaglauca, GCMS, Opportunistic infections, Staphylococcusaureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Candidaalbicans,
[1]. PatilManasi S. and Gaikwad D. K. A Critical Review on Medicinally Important Oil Yielding Plant Laxmitaru (SimaroubaglaucaD.)Department of Botany, Shivaji University, Kolhapur16004 (MS) India
[2]. KhalingMikawlrawng , SandeepKaushik , Anand Kumar Pushker , Suresh Kumar , MoirangthemKameshwor Singh , and GurumayumSuraj Sharma, Comparative in vitro antifungal activities of SimaroubaglaucaagainstFusariumoxysporumandAspergillusparasiticus, Journal of Medicinal Plants Studies,Vol. 2 (3)2004.
[3]. Iasmine A.B.S. Alvesa, Henrique M. Mirandab, Luiz A.L. Soaresa,b, Karina P. Randaua,,Simaroubaceae family: botany, chemical composition andbiological activities, Brazilian journal of pharmacognosy
[4]. Shetonde O. Mihigo,MavongaTekayela1 , Ishmael B. MasesaneandKwengaSichilongo.Preliminarygc-ms profiling and anti-bacterial potential of two congolese invasive weeds, Synedrellanodiflora (l.) gaertn. andAspiliakotschyi (schultzbip.) oliv. (asteraceae),International journal of chemistry and aquatic sciences (ijca), vol 2, issue 1, 2016.
[5]. Shetonde O. Mihigo,LuvengaNdombele, Ishmael B. Masesane, and K. Sichilongo Preliminary GC-MS Profiling and Anti-bacterial activity Investigation of Acanthospermumhispidum DC (Asteraceae) International journal of chemistry and aquatic sciences (IJCA), Vol 1, Issue I, June-July,2015
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| Paper Type | : | Research Paper |
| Title | : | Genotypic Distinctions of Variability of Biochemical Composition of Fruits of Vacciniaceae Species under Conditions of Belarus |
| Country | : | |
| Authors | : | Z.A.Rupasova || А.P.Yakovlev || V.V.Titok || V.N.Reshetnikov || T.I.Vasileuskaya || N.B.Krinitskaya || E.V.Tishkovskaya || A.A.Volotovich |
Abstract: Result of research of quantitative characteristics of biochemical composition of fruits of 30 taxons of 3 Vacciniaceae species (such as V. covilleanum Butkus et Plishka (highbush blueberry), V. vitis-idaea L. (lingonberry) and O. macrocarpus (Ait.) Pers. (cranberry)) inter-specific distinctions of a degree of stability of its separate components to complex influence of meteorological factors are revealed by 32 parameters (traits) describing the contents in fruits of some organic acids, carbohydrates, phenolic compounds, terpenoids and major mineral elements are presented. The cultivars possessing by the greatest and accordingly by the least levels of dependence on abiotic factors are identified.
Keywords:biochemical composition, genotypic variability, Highbush blueberry (V. covilleanum Butkus et Plishka), lingonberry (V. vitis-idaea L.), cranberry (O. macrocarpus (Ait.) Pers.)
[1] Ermakov A.I., Arasimovich V.V., Yarosh N.P., Peruanskij Yu.V., Lukovnikova G.A., Ikonnikova M.I., 1987 Methods of biochemical research of plants. 430 p. (In Russian).
[2] Fomenko K.P., and Nesterov N.N., 1971 Technique of definition of nitrogen, phosphorus and potassium in plants from one assay. Chemistry in agriculture 10: 72-74. (In Russian).
[3] GOST 28561-90, 2011. Fruit and vegetable products. Methods for determination of total solids or moisture. pp. 75-85. Standartinform Publishing, Moscow. (In Russian).
[4] Kalebin M. I, and Kolesnik A.A., 1949 Research of fresh fruits, vegetables and products of their processing. In: Tserevitinov F.V. Research of foodstuff, pp. 218-245, Gostorgizdat Publishing, Мoscow, (In Russian).
[5] Кurlovich T.V., 2007 Cranberry, blueberry, cowberry. 200 p, UNION-public Publishing, Moscow (In Russian).
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| Paper Type | : | Research Paper |
| Title | : | In-Vitro Antibacterial Effect of Crude Extract of Chromolaena Odorata Leaves on Wound Isolates |
| Country | : | Nigeria. |
| Authors | : | Nwachukwu, Ifeanyi || Aliga, Chinanu || Upabi, Chibueze F. || Ukogo, Ifeoma |
Abstract:Medicinal plants have played an important role in prevention and control of diseases. The abundance of plants on the earth's surfaces has led to an increasing interest in the investigation of different extracts obtained from traditional plant as potential sources of new antimicrobial agents. This study was carried out to determine the antibacterial effect of the crude extract of Chromolaena odorata leaves on some wound isolates (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella species). The result shows that the crude extract of Chromolaena odorata leaves was effective on the isolated bacteria, however the control drug Ciprofloxacin had greater inhibition on the tested organism than the plant extracts. The zone of inhibition of crude extract of Chromolaena odorata leaves on S. aureus ranged from 7mm - 18mm, Klebsiella species ranged from 6mm - 17mm, Pseudomonas aeruginosa 5mm -15mm and Escherichia coli 5mm-13mm while the control drug (ciprofloxacin) ranged from16mm-20mm..........
Keywords: Antibacterial effect, Chromolaena odorata, Traditional plant, Crude extract, Medicinal plants.
[1]. Alisi, C. S., Nwaogu, L. A., Ibegbulem, C. O. and Ujowundu, C. U. (2011). Antimicrobial Action of Methanol Extract of Chromolaena Odorata-Linn is Logistic and Exerted by Inhibition of Dehydrogenase Enzymes. Journal of research in Biology, 3: 209-216.
[2]. Anwannil, H. G. and Atta, R. (2006). Trends in ethnopharmacology. J. Ethnopharmacol, 100: 43 – 49
[3]. Azoro, C. (2000). Antibacterial activity of crude extract of Azadirachita indica on Salmonella typhi. World Journal of Biotechnology, 3:347-351
[4]. Bamba, D., Bessiere, J. M., Marion, L., Pelissier, Y. and Fouraste, I. (1993). Essential oil of Eupatorium odoratum. Plant Med., 59: 184-185.
[5]. Benkeblia, N. (2004). Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and Garlic (Allium sativum). Lebensmwiss u-Technol., 37: 263-268..
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| Paper Type | : | Research Paper |
| Title | : | Evaluation of Antdiabetic, Hypocholesterolemic of Pomegranate (Punica Granatum L.) Juice Powders and Peel Powder Extracts In Male Albino Rats |
| Country | : | Egypt |
| Authors | : | Mona Mohamed Abd El Mageid || Nadia Abdel Rahman Salama || Mahmoud Abd-Allah Mouhamed Saleh. || Hossam Mahmoud Abo-Taleb |
Abstract: Punica granatum L., commonly known as pomegranate and belonging to the family of Punicacceae, is a unique plant. Two varieties of pomegranate including Manfaloty and Wonderful were used in this study. The juice powders (JP) subjected to freeze-drying and peel powder extracts (PPE) of the two varieties were used as hypoglycemic and hypocholesterolemic agents by using albino rats. The juice powder was used in two different concentrations (1 and 1.5 g/kg/day/ rat), meanwhile the peel powder extract was used in only one concentration (0.3 g/kg/day/rat). Results show the effect of JP and PPE on the glucose level of diabetic rats. The superior effect was with G6 rats administrated with 0.3 g MPE/kg/day/rat (Manfaloty peel extract) followed by G9 0.3 g WPE/kg/day/rat (Wonderful peel extract). On the other hand results show the effect of JP and PPE on the level of total cholesterol, LDL, HDL and triglycerides in rats serum as hypocholesterolemic agents. These results show that G5 (HFD+1%cholesterol+300mgMPE/kg /day/ rat) recorded the best results of all the aforementioned experiments, this could be attributed to the phenolic content in peel powder extract of pomegranate.
[1]. R. Longtin. The pomegranate: nature's power fruit? J. Natl Cancer Inst 95, 2003, 346–348.
[2]. I. A. T. M. Meerts, C. M. Verspeek-Rip, C. A. F. Buskens, H. G. Keizer, J. Bassaganya-Riera, Z. E. Jouni, A. H. B. M. van Huygevoort, F. M. van Otterdijk, and E. J.van de Waart. Toxicological evaluation of pomegranate seed oil. Food Chem Toxicol, 47(6), 2009, 1085–1092.
[3]. A. Fadavi, M. Barzegar, and H. M. Azizi. Determination of fatty acid and total lipid content in oilseed of 25 pomegranates varieties grown in Iran. J. Food Comp Anal, 19, 2006, 676–680.
[4]. FAOSTAT-FAO . Statistical database. Food and Agriculture Organization of the United Nations, Codex Alimentarius Commision. http://www.fao.org., June 2014.
[5]. O. M. Imsail, R. A. A. Younis, A. M. Ibrahim. Morphological and molecular evaluation of some Egyptian pomegranate cultivars, African journal of Biotechnology, 13(2), 2014, 226-237..
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| Paper Type | : | Research Paper |
| Title | : | A Review on Hyphenated Separation Techniques Used in Pharmaceutical Analysis |
| Country | : | India |
| Authors | : | Devi Thamizhanban || TuljaGampa Rani || Patlolla Pravalika |
Abstract: Hyphenated techniques combine chromatographic and spectral methods to exploit the advantage of both. Chromatography produces pure or nearly pure fractions of chemical components in a mixture.The development of the pharmaceuticals brought a revolution in human health. Pharmaceuticals would serve their intent only if they are free from impurities and are administered in an appropriate amount. To make drugs serve their purpose various chemical and instrumental methods were developed at regular intervals which are involved in the estimation of drugs.The review of hyphenated technique includes various techniques which are used nowadays for analysis. Chromatographic techniques GC,LCetc.........
[1]. Sweedler, J.V. The continued evolution of hyphenated instruments. Analytical &Bio-analytical Chemistry, 2002, 373, 321–322
[2]. Albert, K. Hyphenated techniques. . Analytical &Bioanalytical Chemistry, 2002, 372, 25–26
[3]. Bruins, A. P.; Covey, T. R.; Henion, J. D., Ion spray interface for combined liquidchromatography-atmospheric pressure ionization mass spectrometry. Analytical Chemistry. 1987, 59,2642-2646.
[4]. Nishino, I., Fujitomo, H. &Umeda, T. (2000) J. Chromatogr. B: Biomedical Sciences and Applications,749(1), 101.
[5]. Hirschfeld, T. 1980. The hy-phen-ated methods. Anal. Chem. 52(2): 226–232.
[6]. Dressler, V.L., Antes, F.G., Moreira, C.M., Pozebon, D., and Duarte, F.A. 2011. As, Hg, I, Sb, Se and Sn speciation in body fluids and biological tissues using hyphenated-ICP-MS techniques: A review. Int. J. Mass Spectrom. 307(1-3): 149–162..
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| Paper Type | : | Research Paper |
| Title | : | A Validated Bioanalytical Method for Determination of Glimepiride in Human Plasma Using UPLC-MS/MS |
| Country | : | Egypt |
| Authors | : | Magda M. Ayad || Mervat M. Hosny || Heba M. El-Sayed || Mohamed G. Elwan |
Abstract: In the present work, a sensitive and rapid ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) method was developed and validated for the simultaneous determination of glimepiride (GPD) in human plasma using GPD-d5 as the internal standard (IS). The analyte was separated on an Acquity UPLC BEH C18 column with an isocratic elution system composed of acetonitrile and 0.1 % ammonium formate (70:30) at a flow rate of 0.30 mL/min. GPD was eluted at 1.00 min. Mass spectrometric detection was performed on a triple quadrupole (TQD) mass spectrometer operated in positive electrospray ionization (ESI) mode. The Multiple Reaction Monitoring (MRM) transitions were m/z 491.188→126.009 and m/z 496.188→ 131.009 for GPD and GPD-d5, respectively. The method was validated over a dynamic concentration range of (1– 100) ng/mL...........
Keywords: glimepiride, human plasma, UPLC–MS/MS.
[1]. M. Massi-Benedetti, Glimepiride in type 2diabetes mellitus, a review of the worldwide therapeutic experience, Clinical Therapeutics, 25(3),2003, 799–816.
[2]. R. K. Campbell,Glimepiride: role of a new sulfonylurea in thetreatment of type 2 diabetes mellitus,The Annals of Pharmacotherapy,32(10),1998, 1044–1052.
[3]. E. Draeger, Clinical profile of glimepiride, Diabetes Research and Clinical practice, 28, 1995, S139-146.
[4]. I. U. Khan, F. Aslam, M. Ashfaq, and M. N. Asghar,Determination of glimepiride in pharmaceutical formulations using HPLC and first-derivative spectrophotometric methods, Journal of Analytical Chemistry, 64(2), 2009, 171-175.
[5]. M. R. Regalla and T. V. Ravindranath,Method development and validation for simultaneous estimation of metformin, glimepiride and atorvastatin in combined tablet dosage form by UPLC,World Journal of Pharmacy and Pharmaceutical Sciences, 2(6), 2013, 5229-5347.
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| Paper Type | : | Research Paper |
| Title | : | TNK1 Deficiency Inhibited Cell Survival, Clonogenic Assay and Effects Expression Levels of EMT Markers |
| Country | : | China |
| Authors | : | Alaulddin Hazim Mohammed |
Abstract: PRC is a part of the male reproductive system that helps make and store seminal fluid. In adult men, a typical prostate is about 3 centimetres long and weighs about 20 grams. It is located in the pelvis, below the urinary bladder and in front of the rectum. PRC is considered a malignant tumour because it is a mass of cells that can invade other parts of the body. PRC most commonly metastasizes to the bones, lymph nodes, and may into the rectum, bladder and lower ureters after local progression. The route of metastasis to the bone is thought to be venous as the prostatic venous plexus drairs into the prostate connecting with the vertebral veins. PRC is a zinc-accumulating, citrate-producing organ.........
Keywords: PRC / TNK1 / EMT / MTT / PC-3 cells.
[1]. Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray, F. Cancer Incidence and Mortality Worldwide: IARC Cancer Base GLOBOCAN. 2012, 1, 11.
[2]. José Expósito, Isabel Linares, Isabel Castillo, Miguel Martínez, Pilar Vargas, Ismael Herruzo, José Antonio Medina, Amalia Palacios, Eloísa Bayo, Francisco Peracaula, Javier Jaén, José Antonio Sánchez, and María José Ortiz. Evaluation of the utilization of external radiotherapy in the treatment of localized prostate cancer in Andalusia, Spain. Radiat Oncol. 2015, 10, 265.
[3]. Chamie K, William SB, Hu JC. Population-based assessment of determining treatments for prostate cancer. JAMA Oncol. 2015, 1, 60-67.
[4]. Heidenreich A1, Bastian PJ2, Bellmunt J3, Bolla M4, Joniau S5, van der Kwast T6, Mason M7, Matveev V8, Wiegel T9, Zattoni F10, Mottet N11; European Association of Urology. Guidelines on prostate cancer. Arnhem (The Netherlands): European Association of Urology (EAU). 2014, 65, 467-479.
[5]. Male Genitals - Prostate Neoplasms". Pathology study images. University of Virginia School of Medicine. 2011. http://www.med-ed.virginia.edu/courses/path/gu/prostate3.cfm
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| Paper Type | : | Research Paper |
| Title | : | Ameliorative Effect of Aqueous Leaf Extract of Tectona Gondis on Egg Yolk Induced Heart and Blood Vessels Damage in Adult Wistar Rats |
| Country | : | Nigeria. |
| Authors | : | EHIMIGBAI, Agbonluai Richard .O || MIEHWEE, Goodluck .O. || EHI-OMOSUN, Mabel |
Abstract: The aim of this study was to determine the effect of aqueous extract of Tectona gondis leaf on egg yolk induced heart and blood vessel toxicity in adult wistar rats. A total of 35 (thirty five) adult wistar rats weighing between 200 g to 270 g were divided into 5 groups of seven rats per group. Group A rats were placed on normal diet only while Group B rats received 300 mg/ kg body weight / day (BWT/D) of egg yolk. Group C rats received 300mg / kg BWT/D of Tectona gondis leaf. Group D rats received 300 mg/ kg BWT/D of egg yolk and 300mg / kg BWT/D of T. gondis leaf Group E rats received 300 mg/ kg BWT/D of egg yolk and 500mg / kg BWT/D of T. gondis leaf and all the dosage were given for 30 days via orogastric administration. The biochemical result revealed that group B showed marked increase..........
Keywords: Tectona gondis, Superoxide dismutase, Blood vessel, Catalase, Cholesterol, Heart
[1]. Mukerjee, P.K. Exploring botanicals in Indian systems of medicine-regulatory perspectives, Clinical Research and Regulatory Affairs. 20, 2003, 249–264.
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[5]. Ragasa, C.Y., Lapina, M.C., Lee, J.J., Mandia, E.H., and Rideout, J.A. Secondary metabolites from Tectona philippinensis, Natural Product Research.22,2008, 820–824..
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| Paper Type | : | Research Paper |
| Title | : | Method Development and Analytical Method Validation of Carvedilol by High Performance Liquid Chromatography |
| Country | : | Pakistan |
| Authors | : | Abdullah || Waqas Jamil || Hira Shariq || Jawaid Ahmed Siddiqui |
Abstract: The goal of this study was to develop an easy, specific, accurate, precise and sensitive reversed-phase high performance liquid chromatographic (RP-HPLC) analytical method for the rapid quantitative determination of Carvedilol. Carvedilol is widely used in the early treatment of mild to severe congestive heart failure (CHF) and high blood pressure as it is a nonselective beta blocker/alpha-1 blocker. The chromatographic separation was carried out on Shimadzu 20-A series equipped with solvent delivery pump LC-20AT,autosampler SIL-20ACHT, PDA detector SPD-M20A and degassing unit. The HPLC column was Hibar® (250mm x 4.6mm, 5μm)..........
Keywords: Beta Blockers, Carvedilol, ICH, Method Validation, RP-HPLC
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