Abstract: Aflatoxin contamination of grain has continued to pose a significant threat to sustainable food security and trade worldwide. In the field, there are incidences of varying contamination levels in grain within the same niche. We hypothesize that the variation could be due to fungal species interaction at the kernel level. Seventeen isolates (14 Aspergillus flavus and 3 Aspergillus parasiticus) from Eastern Kenya were selected and confirmed for toxigenicity using Dichlorvos-Ammonia method, then cultured based on their isolation frequencies and co-existence in nature. The fungi were co-cultured using maize kernels as growth substrate, which was then used to estimate aflatoxin produced in a competitive ELISA. A one-sample two-tailed t-test was carried out to determine......
Keywords: Toxigenicity; aflatoxins; fungal interactions; Aspergillus; Dichlorvos-Ammonia
[1]. P. Bhatnagar-Mathur, S. Sunkara, M. Bhatnagar-Panwar, F. Waliyar, and K.K. Sharma, Biotechnological advances for combating Aspergillus flavus and aflatoxin contamination in crops, Plant Science, 234, 2015, 119-132.
[2]. L. Lewis, M. Onsongo, H. Njapau, H. Schurz-Rogers, G. Luber, S. Kieszak, J. Nyamongo, L. Backer, A.M. Dahiye, and A. Misore, Aflatoxin contamination of commercial maize products during an outbreak of acute aflatoxicosis in eastern and central Kenya, Environmental Health Perspectives, 113(12), 2005, 1763.
[3]. J.H. Daniel, L.W. Lewis, Y.A. Redwood, S. Kieszak, R.F. Breiman, W. Dana, C. Bell, J. Mwihia, G. Ogana, S. Likimani, M. Straetemans, and M.A. McGeehin, Comprehensive assessment of maize aflatoxin levels in eastern Kenya, 2005-2007, Environmental Health Perspectives, 119(12), 2011, 1794-1799.
[4]. M.A. Obonyo, and E.N. Salano, Perennial and seasonal contamination of maize by aflatoxins in eastern Kenya, International Journal of Food Contamination, 5(1), 2018, 6.
[5]. R. Bandyopadhyay, A. Ortega-Beltran, A. Akande, C. Mutegi, J. Atehnkeng, L. Kaptoge, A. Senghor, B. Adhikari, and P. Cotty, Biological control of aflatoxins in Africa: current status and potential challenges in the face of climate change, World Mycotoxin Journal, 9(5). 2016, 771-789..