Series-1 (May – June 2026) May – June 2026 Issue Statistics
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| Paper Type | : | Research Paper |
| Title | : | The CRISPR-Cas9 Paradigm: A Multidimensional Analysis Of Mechanistic Precision, Therapeutic Potential, And Socio-Ethical Governance In Modern Biotechnology |
| Country | : | |
| Authors | : | xxx |
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: | 10.9790/ 264X-1203010108  |
Abstract: The discovery of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) platform is a paradigm shift in the background of genomic architecture and molecular therapeutics. Though Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs) provided initial capabilities in the area of gene-editing, they were commonly constrained by their high prices, intricate design, and the lack of throughput. The paper includes an informative analytical inquiry into the CRISPR-Cas9 system, analyzing its mechanistic peculiarities, its disruptive possibilities in biomedical and agricultural.....
[1].
Adli, M. (2018). The CRISPR Tool Kit For Genome Editing And Beyond. Nature Communications, 9(1), 1-13.
[2].
Alberts, B., Johnson, A., Lewis, J., Morgan, D., Raff, M., Roberts, K., & Walter, P. (2022). Molecular Biology Of The Cell (7th Ed.). W. W. Norton & Company.
[3].
Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., ... & Liu, D. R. (2019). Search-And-Replace Genome Editing Without Double-Strand Breaks Or Donor DNA. Nature, 576(7785), 149-157.
[4].
Baltimore, D., Berg, P., Botchan, M., Carroll, D., Charo, R. A., Church, G., ... & Witkowski, J. A. (2015). A Prudent Path Forward For Genomic Engineering And Germline Gene Modification. Science, 348(6230), 36-38.
[5].
Charpentier, E., & Doudna, J. A. (2013). Rewriting A Genome. Nature, 495(7439), 50-51
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| Paper Type | : | Research Paper |
| Title | : | Impact Of Drying And Lacto-Fermentation On The Quality Of Tomato Fruits |
| Country | : | Congo |
| Authors | : | Okondza Nohya Delvina || Nkeletela Laurette Brigelia || Louyindoula Auguste || Bokouango Nessy Noëlie || Mabossy-Mobouna Germain || Mananga Vital |
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: | 10.9790/ 264X-1203010915 |
Abstract: Background : The overall objective of this study was to evaluate the effect of several preservation methods on the physicochemical quality of tomato fruits. The experiment focused on two preservation techniques: drying (at 50, 55, and 60°C) and lacto-fermentation. The physicochemical parameters studied were pH, titratable acidity, Brix degree, water content, protein content, and ash content. The results showed that the ideal temperature for drying tomato fruits is 50°C. Indeed, the physicochemical parameters studied showed that at 50°C, the physicochemical...
Key Word: Tomato, preservation, drying kinetics, lacto-fermentation
[1].
EKISSI AC, KOUAME KB, BEUGRE GA, KATI-COULIBALY S. Improvement Of The Shelf Life Of F1 (Boomerang) Tomato Fruits Using Traditional Preservation Techniques. Afrique Science 2021 19(4) 159 – 173. French.
[2].
Ali MY, Sina AAI, Khandker SS, Neesa L, Tanvir EM, Kabir A, Khalil MI, Gan, SH Nutritional Composition And Bioactive Compounds In Tomatoes And Their Impact On Human Health And Disease: A Review. Foods, 2021;10.45.
Https://Dx.Doi.Org/10.3390/Foods10010045
[3].
Seybold C, Frohlich K, Britsch R, Otto K, Bohm VJ Chnages In Contents Of Carotenoids And Vitamin E During Tomato Processing. Journal Of Agricultural And Food Chemistry, 2004 52(23), 7005-7011.
[4].
Bezabih E, Afari V, Nenguwo N, Ayana A, Kebede D, Mohammed H. Characterization Of Pre And Postharvest Losses Of Tomato Supply Chain In Ethiopia. Agriculture And Food Security, 2017; 6:3, 11p
[5].
Atmani, K., Bakhouche, H. 2018. Comparative Study Of Microwave And Oven Drying Of Tomatoes (Lycopersicon Esculentum Mill). Master's Thesis. A. Mira University – Bejaia, Algeria. P. 88.
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| Paper Type | : | Research Paper |
| Title | : | AI Based Multi-Omics Analysis And Structure-Based Drug Discovery Pipeline Targeting Kras4a Mutations For Precision Oncology |
| Country | : | India |
| Authors | : | Sonali || Uma Kumari |
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: | 10.9790/ 264X-1203011624 |
Abstract: Overall analysis presents an integrated computational framework for precision oncology targeting KRAS4A mutations in Non-Small Cell Lung Cancer (NSCLC). The workflow combines Next-Generation Sequencing (NGS), Computer-Aided Drug Design (CADD), Artificial Intelligence (AI), and bioinformatics tools to systematically identify and evaluate potential therapeutic targets. Structural analysis of KRAS4A (PDB ID: 9GGY) revealed a stable conformation with a well-defined binding pocket between switch I and switch II regions. Sequence similarity and evolutionary relationships were assessed using sequence similarity and protein embedding....
Key Word: KRAS4A; NSCLC; NGS; CADD; Artificial Intelligence; ML; Molecular Docking; Precision Oncology; Biopython
[1]. Corral De La Fuente E, Olmedo Garcia ME, Gomez Rueda A, Lage Y, Garrido P. Targeting KRAS In Non-Small Cell Lung Cancer. Front Oncol. 2022 Jan 10;11:792635. Doi: 10.3389/Fonc.2021.792635. PMID: 35083149; PMCID: PMC8784727.Mechanisms And Current Advances In Treating KRAS-Mutated Lung Cancer
[2]. Zhao, R., Shu, Y., Xu, W. Et Al. The Efficacy Of Immunotherapy In Non-Small Cell Lung Cancer With KRAS Mutation: A Systematic Review And Meta-Analysis. Cancer Cell Int 24, 361 (2024). Https://Doi.Org/10.1186/S12935-024-03498-9
[3].
Ritu, K., Kumar, P., Singh, A. Et Al. Untangling The KRAS Mutated Lung Cancer Subsets And Its Therapeutic Implications. Mol Biomed 2, 40 (2021). Https://Doi.Org/10.1186/S43556-021-00061-0
[4]. Ghosh S, Bhuniya T, Dey A, Koley M, Roy P, Bera A, Gol D, Chowdhury A, Chowdhury R, Sen S. An Updated Review On KRAS Mutation In Lung Cancer (NSCLC) And Its Effects On Human Health. Appl Biochem Biotechnol. 2024 Jul;196(7):4661-4678. Doi: 10.1007/S12010-023-04748-8. Epub 2023 Oct 28. PMID: 37897621.
[5].
He, Q. Et Al. (2025) ‘First-Line Treatments For KRAS-Mutant Non-Small Cell Lung Cancer: Current State And Future Perspectives’, Cancer Biology & Therapy, 26(1). Doi: 10.1080/15384047.2024.2441499..
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| Paper Type | : | Research Paper |
| Title | : | NGS And Structure Based Drug Designing Of Natural Compound Against Drug Resistant EGFR In Non-Small Cell Lung Cancer |
| Country | : | India |
| Authors | : | Riyaz Gurung || Uma Kumari |
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: | 10.9790/ 264X-1203012534 |
Abstract: Mutations in Epidermal Growth Factor Receptor drives disease progression and treatment resistance in Non-small Cell Lung Cancer. EGFR is a well-documented therapeutic target for management of NSCLC. Our research combines Next Generation Sequencing (NGS) and Structure Based Drug Design (SBDD) approaches to identify natural compounds as potential therapeutical. In our study, we targeted the triple mutated EGFR kinase domain (L858R T790M/C797S) using its crystal structure (PDB ID: 9D3W) to design EGFR tyrosine kinase inhibitors. NGS examined...
Key Word: Next-Generation Sequencing (NGS), Structure Based Drug Design (SBDD), Natural products, EGFR Mutants, Non-small Cell Lung Cancer (NSCLC), Molecular Docking, Targeted Therapy, Precision Medicine
[1].
Bajaj, T., & Kumari, U. (2025). Issue 3 Www.Jetir.Org (ISSN-2349-5162). In JETIR2503614 Journal Of Emerging Technologies And Innovative Research (Vol. 12). Www.Jetir.Org
[2].
Bandbe, T., Saikia, J., & Kumari, U. (N.D.). NGS Analysis Human Papillomavirus Type 18 E2 DNA-Binding Domain Bound To Its DNA Target With Biopython.
[3].
Bindod, H. V., Hatwar, P. R., Bakal, R. L., & Dafe, V. N. (2025). A Comprehensive Review Of Cancer: Types, Pathophysiology, Diagnosis And Treatments. Journal Of Drug Delivery And Therapeutics, 15(5), 114–122. Https://Doi.Org/10.22270/Jddt.V15i5.7104
[4].
Ferro, A., Marinato, G. M., Mulargiu, C., Marino, M., Pasello, G., Guarneri, V., & Bonanno, L. (2024). The Study Of Primary And Acquired Resistance To First-Line Osimertinib To Improve The Outcome Of EGFR-Mutated Advanced Non-Small Cell Lung Cancer Patients: The Challenge Is Open For New Therapeutic Strategies. In Critical Reviews In Oncology/Haematology (Vol. 196). Elsevier Ireland Ltd. Https://Doi.Org/10.1016/J.Critrevonc.2024.104295.
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| Paper Type | : | Research Paper |
| Title | : | Role Of Sialyltransferases In Tumor Progression And Cancer Metastasis: A Comprehensive Review Article |
| Country | : | India |
| Authors | : | Ms. Riya |
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: | 10.9790/ 264X-1203013540 |
Abstract: Cancer progression is closely associated with alterations in glycosylation, particularly hyper sialylation mediated by sialyltransferases (STs). Sialylation is a critical post-translational modification involving the transfer of sialic acid residues to glycoproteins and glycolipids, thereby regulating cellular communication, adhesion, immune recognition, and metastatic behavior. Aberrant expression of sialyltransferases has emerged as a hallmark of malignant transformation and contributes significantly to tumor growth, invasion, immune evasion, metastasis, and therapeutic...
Key Word: Sialyltransferases, Cancer Metastasis, Glycosylation, Hyper sialylation, Tumor Progression, Glycobiology, Therapeutic Targets, Siglecs, Selectins
[1].
Pietrobono S, Stecca B. Aberrant Sialylation In Cancer: Biomarker And Potential Target For Therapeutic Intervention? Cancers (Basel). 2021;13(9):2014. Doi:10.3390/Cancers13092014.
[2].
Perez SJLP, Fu CW, Li WS. Sialyltransferase Inhibitors For The Treatment Of Cancer Metastasis: Current Challenges And Future Perspectives. Molecules. 2021;26(18):5673. Doi:10.3390/Molecules26185673.
[3].
Bhide GP, Colley KJ. Sialylation Of N-Glycans: Mechanism, Cellular Compartmentalization And Function. Histochem Cell Biol. 2017;147(2):149–174. Doi:10.1007/S00418-016-1520-X.
[4].
Szabo R. New Avenues In The Design Of Sialyltransferase Inhibitors. 2017.
[5].
Chen JH. Tumour Invasion And Metastasis. Tzu Chi Med J. 2002;14(1):1–5. Doi:10.1016/S0959-8049(01)80498-6.
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| Paper Type | : | Research Paper |
| Title | : | Helicobacter pylori Infection and miRNA-139 Dysregulation in Hepatocellular Carcinoma: A Clinical Biochemical Case–Control Study |
| Country | : | Egypt |
| Authors | : | Tarek S. Ebrahim || Ahmed M. El-Hilaly || Heba A. Elshahawy || Tarek M. Okda |
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: | 10.9790/ 264X-1203014154 |
Abstract: Background and aim: Hepatocellular carcinoma (HCC) remains one of the leading causes of cancer-related mortality worldwide. Emerging evidence suggests that Helicobacter pylori infection may contribute to hepatocarcinogenesis through chronic inflammation, immune dysregulation, oxidative stress, and modulation of molecular signaling pathways. However, the clinical and molecular relationship between H. pylori infection and HCC progression remains incompletely understood. Methods: A prospective case–control study was conducted on 180 participants recruited from the Gastrointestinal Surgery Center, Mansoura University, Egypt, between 2023 and 2024. Participants were divided into three groups: HCC patients with H. pylori infection (n=60), HCC patients without H.......
Key Word: Hepatocellular carcinoma, Helicobacter pylori, miRNA‑139, AFP, liver cancer, inflammation, biomarkers.
[1].
CA: A Cancer Journal for Clinicians Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global cancer statistics 2023: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2024;74(1):1–35.
[2].
Nature Reviews Disease Primers Llovet JM, Kelley RK, Villanueva A, Singal AG, Pikarsky E, Roayaie S, et al. Hepatocellular carcinoma. Nat Rev Dis Primers. 2021;7(1):6.
[3].
Villanueva A. Hepatocellular carcinoma. N Engl J Med. 2019;380(15):1450–62.
[4].
Forner A, Reig M, Bruix J. Hepatocellular carcinoma. Lancet. 2018;391(10127):1301–14.
[5].
Elgharably A, Gomaa AI, Crossey MM, Norsworthy PJ, Waked I, Taylor-Robinson SD. Hepatitis C in Egypt – past, present, and future. Int J Gen Med. 2017;10:1–6.
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| Paper Type | : | Research Paper |
| Title | : | Plant Growth Promoting and Endophytes Bacteria: Mechanisms, Applications, and Future Perspectives for Sustainable Agriculture |
| Country | : | India |
| Authors | : | Mohd Arshan || Abin Mani || Moumita Majumdar || Shravan Paraste || Ayaz khan || Priti Kushwaha |
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: | 10.9790/ 264X-1203015565 |
Abstract: Background: Ensuring long-term food safety and security is a serious global issue, mainly in developed nations where the constancy of food supply is under threat from an uncertain agriculture climate change. Rapid population growth and decreasing soil fertility is unnecessary dependence on chemical fertilizers are the major global concerns in sustainable agriculture. The different environmental stresses such as waterlogging, drought, saline, ultraviolet (UV) radiation, pH (acidic/sodic soils) and temperature variations, which have a thoughtful impact on plant growth and quality......
Key Word: Plant Growth-Promoting Bacteria (PGPB), Nitrogen fixing bacteria, Sustainable Agriculture, Biofertilizers, Biopesticides, Endophytic bacteria.
[1].
Farooq, M. S., Uzair, M., Raza, A., Habib, M., Xu, Y., Yousuf, M., ... & Ramzan Khan, M. (2022). Uncovering the research gaps to alleviate the negative impacts of climate change on food security: a review. Frontiers in plant science, 13, 927535.
[2].
Schmidhuber, J., & Tubiello, F. N. (2007). Global food security under climate change. Proceedings of the national academy of sciences, 104(50), 19703-19708.
[3].
Ahmad, M. F., Ahmad, F. A., Alsayegh, A. A., Zeyaullah, M., AlShahrani, A. M., Muzammil, K., ... & Hussain, S. (2024). Pesticides impacts on human health and the environment with their mechanisms of action and possible countermeasures. Heliyon, 10(7).
[4].
Farhan, M., Sathish, M., Kiran, R., Mushtaq, A., Baazeem, A., Hasnain, A., ... & Moustafa, M. (2024). Plant nitrogen metabolism: balancing resilience to nutritional stress and abiotic challenges. Phyton, 93(3), 581.
[5].
Aktar, W., Sengupta, D., & Chowdhury, A. (2009). Impact of pesticides use in agriculture: Their benefits and hazards. Interdisciplinary Toxicology, 2(1), 1–12.
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| Paper Type | : | Research Paper |
| Title | : | Genome Editing Tools for Enhancing Stress Tolerance in Plants: Advances, Applications, and Future Perspectives |
| Country | : | India |
| Authors | : | Priti kushwaha || Moumita Majumdar || Abin Mani || Mohd Arshan |
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: | 10.9790/ 264X-1203016672 |
Abstract: Globally, agriculture yields have been impacted by climate change and rising environmental challenges. Plants are frequently exposed to various abiotic stresses, such as drought, salinity, extreme temperatures, and heavy metal toxicity, as well as biotic stresses caused by various pathogens and insects. The development of stress tolerant crops through traditional breeding methods is frequently time consuming and constrained by genetic variability. Genome editing technologies have emerged as powerful tools for precise and targeted modification of plant genomes, enabling rapid improvement of stress tolerance traits. Plant biotechnology has been transformed by a number......
Key Word: Genome editing, CRISPR-Cas9, TALENs, ZFNs, Base editing, Prime editing, Abiotic stress, Biotic stress, Crop improvement, Climate resilience
[1]. Du, B., Haensch, R., Alfarraj, S., & Rennenberg, H. (2024). Strategies of plants to overcome abiotic and biotic stresses. Biological Reviews, 99(4), 1524-1536.
[2]. Mansi, M., & Danai, P. (2026). The emerging impact of CRISPR and gene editing on global crop improvement. Transgenic Research, 35(1), 8.
[3]. Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013). ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends in biotechnology, 31(7), 397-405.
[4]. Khalil, A. M. (2020). The genome editing revolution. Journal of genetic engineering and biotechnology, 18(1), 68.
[5]. Fang, Y., & Xiong, L. (2015). General mechanisms of drought response and their application in drought resistance improvement in plants. Cellular and molecular life sciences, 72(4), 673-689.
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| Paper Type | : | Research Paper |
| Title | : | Risk-Based Biocompatibility Evaluation of a Pulmonary Clot Retrieval Device: Cytotoxicity, Skin Sensitization and Skin Irritation |
| Country | : | India |
| Authors | : | Dr. Pramod Kumar Minocha || Dr. Deveshkumar Kothwala || Kamna Pandya || Dr. Brijesh Patel || Aadarsh Tiwari || Krinal Bhandari || Nirav Rana || Parimal Patel |
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: | 10.9790/ 264X-1203017383 |
Abstract: Mechanical thrombectomy medical devices for pulmonary embolism (PE) provide a less invasive way to improve pulmonary perfusion and prevent mortality. The biocompatibility of these devices is essential for reducing undesired biological responses like cytotoxicity, hypersensitive reactions, or irritation. This investigation evaluates the in vitro and in vivo biocompatibility of an innovative pulmonary clot retrieval device constructed out of a nitinol framework with a polymeric coating that complies with ISO 10993 specifications. Cytotoxicity has been assessed by exposing......
Key Word: Pulmonary embolism, pulmonary clot retrieval device, mechanical thrombectomy, ISO 10993, Skin sensitization, Irritation, Cytotoxi
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[2]. Karki, A., Manandhar, L., & Shrestha, S. (2025). Pulmonary Embolism (PE)-Risk Factors, Symptoms, Diagnosis and Treatment. The American Journal of Patient Health Info, 2(1).
[3]. Hartmann, I. J., & Screaton, N. J. (2020). Pulmonary circulation and pulmonary thromboembolism. In Grainger & Allison’s Diagnostic Radiology, 2 Volume Set E-Book. Elsevier.
[4]. Seitler, S., Dimopoulos, K., Ernst, S., & Price, L. C. (2023, December). Medical emergencies in pulmonary hypertension. In Seminars in Respiratory and Critical Care Medicine (Vol. 44, No. 06, pp. 777-796). Thieme Medical Publishers, Inc.
[5]. Corrigan, D., Prucnal, C., & Kabrhel, C. (2016). Pulmonary embolism: the diagnosis, risk-stratification, treatment and disposition of emergency department patients. Clinical and experimental emergency medicine, 3(3), 117.