Volume-8 ~ Issue-3
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| Paper Type | : | Research Paper |
| Title | : | Effects of NaCl and Lime water on the Hypoglycemic and Antioxidant activities of Ocimum gratissimum in Alloxan-induced Diabetic rats |
| Country | : | Nigeria |
| Authors | : | Makinwa T.T., Adiele H., Oyebiyi O.O., Alabi T.D., Sodeinde K. O. |
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: | 10.9790/3008-0830105  |
Abstract: The antidiabetic and antioxidant activities of Ocimum gratissimum (O.G) are well documented. This study was designed to investigate the effects of adding NaCl or lime water to aqueous leaves extract of O.G in alloxan (100mg/kg)-induced diabetic rats. 25 male wistar rats of average weight 180 were divided into 5 groups as; control, diabetic, diabetic treated with O.G (400 mg/kg), diabetic treated with O.G+ NaCl and diabetic treated with O.G + lime water for six weeks. At the end of the experiment, plasma glucose level, Total protein, Albumin and liver ALT, AST, MDA and GSH were determined. The results showed that the aqueous extract of O.G, significantly (P<0.05) reduced the plasma glucose, liver MDA and significantly (P<0.05) increased the liver GSH and ALT of the treated diabetic rats. However these values were found to be significantly (P<0.05) reduced by the addition of NaCl and limewater. While there were no significant (P<0.05) differences in the plasma albumin, total proteins and liver AST in the treated groups as compared with the control. This study showed that the addition of NaCl or lime water to the extracts of O. gratissimum may reduce the hypoglycemic and antioxidant activities associated with O. gratissimum.
Keywords: Antidiabetic, Antioxidant, Lime water, NaCl and Ocimum gratissimum.
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| Paper Type | : | Research Paper |
| Title | : | In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, sargassum wightii |
| Country | : | India |
| Authors | : | Manoj Kumar K., Seema |
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: | 10.9790/3008-0830611 |
Abstract: Polysaccharides of edible algae attracted extensive interest due to their numerous biological activities. Sargassum wightii C. Agardh, belongs to Sargassaceae, is a brown algae in sea shores in Mandapam Rameshwaram and in and aroud areas. The cancer chemopreventive activity of different fractionsof water-soluble polysaccharide extract derived from S.wightii. Estimation of cancer chemopreventive activity, specifically anti-initiation, including the modulation of carcinogen metabolism and the antioxidant capacity,.poly sacharides from Sargassum wightii have a broad spectrum of novel cancer chemopreventive agents. cell-and enzyme- based in vitro assays with markers relevant for measuring inhibition of carcinogenesis during the initiation, promotion, and progression stage. These bioassay systems offer effective identification and evaluation of lead compounds for the development of effective chemopreventive agents and the elucidation of their mechanism of action.
Keywords: Apoptosis, Anti-promoting, Cancer chemo prevention, MTT assay, Sargassum wightii.
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| Paper Type | : | Research Paper |
| Title | : | Parental Attitude towards the use of Contraceptives by Adolescents in Osogbo, Osun State |
| Country | : | Nigeria |
| Authors | : | Oyediran O. Olufemi, Faronbi Joel, Ajibade B. L. |
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: | 10.9790/3008-0831218 |
Abstract: Teenage pregnancy is an issue of concern in the world all over and it results from indiscriminate sex among the adolescents, this may not be unconnected to non usage of contraceptives by this age group due to the attitude of parents about it. Contraceptives are methods of preventing unwanted pregnancy among sexually active couples or adolescents and when effectively utilized, it promotes the health of family member and indirectly improving the health of the nation. The study assessed the parental knowledge about contraceptives, attitude of parents toward provision of contraceptives to adolescents and also found out the appropriate method that parents prefer for adolescents in Osogbo. The study adopted a descriptive design, 400 respondents were selected through a multi stage sampling technique from the two local governments in Osogbo. Data was collected using a validated structured questionnaire. Data was analyzed using descriptive and inferential Statistical Package for Social Sciences.
Keywords: Attitude, Parental, Adolescents, Contraceptives and Use.
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| Paper Type | : | Research Paper |
| Title | : | Shoot tip cryopreservation by vitrification in Kaempferia galanga L. An endangered, overexploited medicinal plant in Tropical Asia |
| Country | : | India |
| Authors | : | T. S. Preetha, A. S. Hemantha Kumar, P. N. Krishnan |
|
: | 10.9790/3008-0831923 |
Abstract: Shoot tips from the established in vitro shoot cultures of Kaempferia galanga L., an endangered medicinal plant were successfully cryopreserved using the vitrification procedure. Effect of concentration of sucrose for preculture, vitrification treatment with PVS2(Plant vitrification solution 2) and recovery medium on cryopreservation of shoot tip meristems has been analyzed. Overnight preculturing of the dissected shoot tips in MS medium containing 0.4 M sucrose, osmoprotection with loading solution for 20 minutes, dehydration with PVS2 for 20 minutes at 0 oC were found to be optimum among the various treatments tested. The cryoprotected shoot tips upon rapid freezing in LN (liquid nitrogen) followed by rapid thawing produced 50-60% survival and 30-40% regeneration rates. Incorporation of GA3 (giberellic acid) in the post-thaw culture medium was essential to induce shoot emergence in the initial phase. Subculturing of the recovered shoot tips in MS medium supplemented with 1.0 mgl-1 BA (6-benzyl adenine) and 0.5 mgl-1 NAA (ƒ¿-naphthaleneacetic acid) resulted plantlet production. The regenerated shoots grew to mature plants were free of any morphogenetic variation upon field transfer.
Keywords: cryopreservation, endangered medicinal plant, long - term conservation, shoot tips, vitrification.
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