Volume-7 ~ Issue-3
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| Paper Type | : | Research Paper |
| Title | : | Isolation, Characterization, Determination of Probiotic Properties of Lactic Acid Bacteria from Human Milk |
| Country | : | India |
| Authors | : | J. R. Kavitha, T. Devasena |
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: | 10.9790/3008-0730107  |
Abstract: Probiotic mean live microorganisms that have beneficial effects on their host's health. Although probiotic strains can be isolated from many sources; for human applications the main criteria is being human origin. Breast milk is an important nutrient source for neonates. Lots of studies showed that this fluid has beneficial effects on the health of neonates. One reason of being beneficial is explaining by the micro flora of human breast milk including beneficial lactic acid bacteria. In this study, isolates were identified by biochemical and molecular characterization and also probiotic properties of lactic acid bacteria, isolated from human milk were investigated. Two of the isolates were observed as potential probiotic. Two of them are bacilli. These isolates showed resistance to stomach pH (pH 3.0), tolerance against 0,3% bile concentration and antimicrobial activity against, Escherichia coli, Bacillus ceruse and Staphylococcus aureus and. After investigation the probiotic properties of these isolates, they were identified by biochemical characterization techniques and molecular identification by using Polymeric chain Reaction (PCR) of 16S ribosomal RNA (rRNA) and 16S sequencing. Two lactobacilli were identified as Lactobacillus rhamnosus (KF477283) and Lactobacillus casei. (KF477282) In the light of this study, it is observed that, human milk is a source of potential probiotic strains.
Keywords: PCR-Polymeric chain reaction, RNA-ribosomal RNA
[1]. Angelis, M., Siragusa, S., Berloco, M., Caputo, L., Settanni, L., Alfonsi, G., Amerio,M., Grandi, A., Ragni, A., and Gobbetti, M. 2006. Selection of potential probiotic actobacilli from pig feces to be used as additives in pelleted feding. Research in Microbiology. 157:792-801.
[2]. Brady, L.J., Gallaher, D.D., Busta, F.F. 2000. The role of probiotic cultures in the prevention of colon cancer. The Journal of Nutrition. 130:410S-414S.
[3]. Bulut, Ç. 2003. Isolation and molecular characterization of lactic acid bacteria from cheese. IYTE Thesis of Ms.
[4]. Çakır, İ. 2003. Determination of some probiotic properties on Lactobacilli and Bifidobacteria. Ankara University Thesis of Ph.D.
[5]. Cardinal, M.J., Meghrous, J., Lacroix, C., Simard, R.E., 1997. Isolation of Lactococcus lactis strain producing inhibitory activity against Listeria. Food Biotechnology11:129.
[6]. Castagliuoluolo, I., Rıegler, M.F., Valenick, L., Lamont, J.T., and Pothoulakis, C. 1999.Saccharomyces boulardii protease inhibits the effects of Clostridium difficile toxins a and b in human colonic mucosa. American Society for Microbiology 67(1):302-307.
[7]. Charteris, W. P., Kelly, P. M., Morelli, L., and Collins, J.K. 1997. Selective detection, enumeration and identification of potentially probiotic Lactobacillus and Bifidobacterium species in mixed bacterial populations. International Journal of Food Microbiology 35:1-27.
[8]. Chou, L.S and Weimer, B. 1999. Isolation and characterization of acid and bile tolerant isolates from strains of Lactobacillus acidophilus. Journal of Dairy Science 82:23- 31.
[9]. Chuayana, Jr. E.L., V. Ponce, C., Rivera, M.R.B., Cabrera, E.C. 2003. Antimicrobial activity of probiotics from milk products. Phil J. Microbiol. Infect. Dis. 32(2):71- 74.
[10]. Chung, H.S., Kim, Y.B., Chun, S.L., and Ji, G.E. 1999. Screening and selection of acid and bile resistant Bifidobacteria. International Journal of Food Microbiology 47:25-32.
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| Paper Type | : | Research Paper |
| Title | : | Effects of Magnesium on Mechanical Properties of Human Bone |
| Country | : | India |
| Authors | : | Raviraj Havaldar, S. C. Pilli, B. B. Putti |
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: | 10.9790/3008-0730814 |
Abstract: The human bone is multiphase material consisting of collagenous matrix interspread with mineral crystals. Magnesium participates in the normal formation and remodelling of bone. Magnesium deficiency has been associated with a number of clinical disorders including osteoporosis. The present investigation focuses on quantitative estimation of magnesium, an inorganic constituent of bone and determination of mechanical strength associated with magnesium namely tensile, compression, bending, torsion and shear loads. In particular, this investigation shows that bending strength in human bone is highly dependent on magnesium content of the bone. A correlation is developed to establish relationship between mechanical strength and magnesium composition. The study shows that quantity of magnesium present in the bone and ageing process are interdependent and are highly correlated.
Keywords: Bone, magnesium, mechanical strength, ageing process..
[2] Mbuyi-Muamba J.-M., Dequeker J., and Gevers G., Biochemistry of Bone, Baillieres Clinical Rheumatology, vol. 2, 1988.
[3] Adams M. A., Kerin A. J., and Bhatia L. S., Experimental determination of stress distribution in articular cartilagr before and after sustained loading, J. of Clinical Biomechanics, vol. 14, pp. 88-96, 1999.
[4] Beason D. P., Dakin G. J., Lopez R. R., and Alonso J. E., Bone mineral density correlates with fracture load in experimental side impacts of the pelvis, J. of Biomechanics, vol. 36, pp. 219-227, 2002.
[5] Anderson J. J. B., Calcium, phosphorus and human bone development, J. Nutr., vol. 126, p. 1153S-1158S, 1996.
[6] Ardeshirpour L., Cole D., and Carpenter T., Evaluation of bone and mineral disorders, J. of Pediatric Endocrinology, vol. 5, pp. 584-598, 2007.
[7] Braillon P. M., and Chotel F., Bone mineral content and soft-tissue assessment in limb segments by dual-energy X-ray absorptiometry, J. of Clinical Densitometry, vol. 6, pp. 149-158, 2003.
[8] Dziedzic-Goclawska A., Ostrowski K., and Michalik J., Decrease of the crystallinity of bone mineral in osteopetrotic rats, Metab. Bone Dis. & Rel. Res., vol. 2, pp. 33-37, 1979.
[9] Rude R. K., Singer F. R., and Gruber H. E., Skeletal and hormonal effects of magnesium deficiency, Am J. Clin Nutr, vol. 28, pp. 1312-141, 2009.
[10] Ebel H., and Guenther T., Magnesium metabolism: A review, J. of Clin. Chem. Clin. Biochem. , vol. 18, pp. 257-270, 1980.
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| Paper Type | : | Research Paper |
| Title | : | Bacterial Isolates and Semen Analysis of Male Partners of Infertile Couples. |
| Country | : | Nigeria. |
| Authors | : | Chika P. Enwuru, Uchenna M. Agbasi, Sarah I. Umeh, Richard C. Egbuobi |
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: | 10.9790/3008-0731520 |
Abstract: BACKGROUND: Seminal tract infections alter the semen quality and affect the sperm parameters of men with varying degree of effect to male fertility. OBJECTIVES: This study was carried out to investigate the presence of infectious agents other than sexually transmitted infections in semen and their relation to semen parameters. METHODS: Five hundred and eighty-seven semen samples of males investigated for infertility were analyzed, inoculated on sterile culture media, and incubated for 24hrs at 37oC. The isolates were identified by standard biochemical tests and their antimicrobial susceptibility determined. RESULTS: Fifty-four percent of the samples had normal sperm concentration, 41.1% had oligospermia, and 4.9% had azoospermia. About 64.7% had normal viscous consistency, 23.0% were high viscous, and 12.3% low viscous. Also, 19.4% had highly motile permatozoa, 51.5% moderately, 27.6% poorly and 1.6% entirely non-motile spermatozoa. About 55.9% of the samples were infected with different nonspecific pathogens. CONCLUSSION: 55.9% of the semen samples were infected and this significantly affected the semen parameters. It is necessary to investigate male partners of infertile couples for infections and appropriate treatment initiated.
Key words: consistency, infection, morphology, Semen, spermatozoa
[1]. Decreases in Male Fertility due to Environmental Factors. Environmental Health Perspectives. Supplement, 101(2): 1993, 65-71
[2]. Male infertility. National Institutes of Health (NIH). Available: http://www.nih.gov/ (accessed 14/03/2013)
[3]. D.R. Franken and O.A. Dada, Does Training Assist Medical Laboratory Scientists with Better Evaluation of Sperm Morphology? African Journal of Reproductive Health 11(1): 2007, 107-112.
[4]. World Health Organization (WHO), Manual for the Standardized Investigation and Diagnosis of the Infertile Couple (Cambridge University Press UK, 1993)
[5]. N.N. Mogra, A.A. Dhruva and L.K. Kothari, Non specific Seminal Tract Infection and Male Infertility: A Bacteriological Study. J. Postgrad. Med; 27: 1981, 99-104.
[6]. M.B. Khalili and M.K. Sharifi-Yazdi, The Effect of Bacterial Infection on the Quality of Human's Spermatozoa. Iranian J. Public Health 30 (3-4): 2001, 119-122.
[7]. I.B. Ivanov, M.D. Kuzmin and V.A. Gritsenko, Micro flora of the Seminal Fluid of Healthy Men and Men Suffering from Chronic Prostatitis Syndrome. International Journal of Andrology 32 (5): 2009, 462-467.
[8]. World Health Organization (WHO) Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction 4th edn.( UK. Cambridge University Press, 1999)
[9]. D. Mortimer, Practical Laboratory Andrology (Oxford University Press, Oxford, 1994, 71-86).
[10]. T.F. Kruger, R. Menkveld, F.S.H. Stender, C. J. Lombard, J. P. van der Merwe, J. A. van Zyl, and K. Smith , Sperm Morphologic Features as a Prognostic Factor in In-Vitro Fertilization. Fertil. Steril 46: 1986, 1118-1123.