Volume-7 ~ Issue-1
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| Paper Type | : | Research Paper |
| Title | : | Analysis of Cow's Urine for Detection of Lipase Activity and Anti-Microbial Properties |
| Country | : | India |
| Authors | : | Saneesh Kumar |
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: | 10.9790/3008-0710108  |
Abstract: There is enormous amount of literature in Ayurveda stating the importance of cow's urine for all purposes, including its importance in our daily life. This research targets on the antimicrobial activity of urine and its biochemical content that can be the key potential factor for its usage as a medicine. A detailed biochemical analysis of cow's urine was done to understand its antibacterial/antifungal properties along with lipase activity which could make it a potentially effective anti-cancer agent. Various micro-organismal plating techniques were applied using Nutrient and Potato Dextrose Agar as the medium for bacterial and fungal growth, to study the inhibitory activity of cow's urine on these organisms. Thin layer chromatography, Volumetric analysis, Spectrophotometric analysis and Tributyrintests were conducted on cow's urine sample to analyze the lipase activity present within the urine content. Statistical comparisons, of the spectra obtained from the Spectrophotometric analysis of urine sample, were made with those already analyzedon various types of lipase activity detectionsfrom previous researches,and similarities were observed in both studies to ascertain the lipase factor potential within cow's urine sample. The tests proved that cow's urine was highly effective in inhibiting bacterial and fungal growth and also a potential natural source of lipase enzyme.
Keywords: Anti-fungal, Ayurveda, Cow's urine, Lipase, Skin-disease.
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Analysis ofCow's Urine for Detection of Lipase Activity and Anti-Microbial Properties.
www.iosrjournals.org 8 | Page
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| Paper Type | : | Research Paper |
| Title | : | Comparative study of sympathetic cardiovascular parameters in overweight, normal and underweight school children in southern Odisha basing on BMI criteria |
| Country | : | India |
| Authors | : | Dr. Debasish Das, Dr. Bipin Bihari Pradhan, Dr. Minati Patnaik |
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: | 10.9790/3008-0710911 |
Abstract: This study was undertaken to investigate and compare the sympathetic cardio vascular parameters in age matched overweight, underweight and normal weight school going boys in southern Odisha. 75 Boys between age group of 12-16 were subjected to study out of which 25 were overweight (BMI>25), next 25 were underweight(BMI<18.5),rest 25 were control group having normal BMI. Cold pressure test and hand grip dynamometer test were performed and blood pressure was measured during and after the tests as measures of cardiovascular parameter. Baseline SBP and MAP were significantly higher in overweight boys & lower in underweight boys. Maximum rise of SBP, DBP & MAP during hand grip dynamometer test were significantly higher in overweight boys & lower in underweight boys. Increase in SBP & MAP from their basal value during cold pressure test were significantly lower in overweight boys & higher in underweight boys. Thus it is concluded that both overweight & underweight boys have derangement of sympathetic cardiovascular function. SBP- Systolic blood pressure, DBP- Diastolic blood pressure , MAP- Mean arterial pressure
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| Paper Type | : | Research Paper |
| Title | : | Potential Biodeteriogens of Indoor and Outdoor Surfaces (Coated With Gloss, Emulsion and Textcoat Paints) |
| Country | : | Nigeria |
| Authors | : | L. O. Odokuma, D. P. Berebon, C. B. Ogbonna |
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: | 10.9790/3008-0711219 |
Abstract: Potential Biodeteriogens of indoor and outdoor surfaces (coated with gloss, emulsion and text coat paints) within the University of Port Harcourt, Nigeria were investigated. Potential Biodeteriogens implicated in deterioration of painted surfaces were bacteria, fungi, microalgae and cyanobacteria. The total heterotrophic bacteria counts and total fungal counts for outdoor and indoor painted surfaces ranged from 2.8 x 106 to 9.00 x 106 cfu/g paint scrape, 1.56 x 104 to 6.6 x 104 cfu/g paint scrape; and 1.1 x 106 to 6.5 x 106 cfu/g paint scrapes, 1.31 x 104 to 9.8 x 104 cfu/g paint scrapes respectively. The result of THB and TF count expressed graphically showed surfaces with increasing order of microbial load: Gloss paints < Text coat paint < Emulsion paints. Predominant bacterial genera isolated from the surfaces include Bacillus (29.0%), Pseudomonas (22.6%), Proteus (19.4%), Serratia (16.1%), Citrobacter (6.5%), Enterobacter (3.2%) and Klebsiella (3.2%). Fungal genera isolated include: Alternaria, Aspergillus , Cladosporium , Fusarium ,Geotrichum , Gleosporium , Penicillum , Rhizopus , Saccharomyces and Stachybotrys . Fungi were the predominant biodeteriogens. Predominant microalgae isolated from the wet painted surfaces include Chorella , Characium , Closterium , Geminella , Oscillatoria , Totrogonnidium and Triceratium . Physicochemistry of various paint surfaces revealed the following: TOC (1.30 – 3.49%), Phosphate (0.39-8.82mg/100g), nitrate (4.64-187.58mg/100g), sulphate (99.78-285.00mg/100g), pH (8.55-9.59), oil and Grease (125.00-285.00mg/100g).Result showed that different consortia of biodeteriogens implicated in indoor and outdoor painted surfaces are dependent on the chemical compositions of the various paints, nature of the coating surfaces and physicochemical parameters influencing the microbial processes. Emulsion surfaces habour most potential biodeteriogens on their surfaces than the other surfaces. There were significant differences (P < 0.05) in the various potential biodeteriogens, categories of painted surfaces, indoor and outdoor surfaces.
Keywords: Biodeteriogens, indoor, outdoor, painted surfaces
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| Paper Type | : | Research Paper |
| Title | : | Estimation of Anti Hbs antibody titer in adults during 5-10 years period following three doses of vaccine |
| Country | : | India |
| Authors | : | Dr. B. S. Mahawal, Dr. N. Bhai, Dr. V. K. Kataria, Dr. N. Gulati, Dr. I. Chandola |
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: | 10.9790/3008-0712023 |
Abstract: Health care professional are a high risk group for Hepatitis B and are advised vaccination against hepatitis B. The protective antibodies induced by Hepatitis B vaccination wane gradually over period of time and may reach very low or even undetectable levels. A cross sectional prospective study was undertaken to investigate the persistence of anti-HBs levels in health care professionals who had been immunized with HBV vaccine and the need for booster doses of the vaccine. In this cross-sectional study, health care professionals (18 -55 yrs of age) who had received full course of HBV vaccination were sampled and tested for anti-HBs from May 2010 to June 2011. Plasma samples were tested for anti-HBs using ELISA. Titer >=10 mIU/mL was considered to be seroprotective. Individuals with titre <10 mIU/mL were advised full three doses of vaccine. A total of 112 samples were collected. Protective antibody levels were detected in 99.9% of subjects one year after vaccination. It decreased to 80.96% by 5 years after vaccination, and further decreased to 46.16% in 10 years after vaccination. Sero protection rates decreased significantly with increasing time from last vaccination due to waning anti-HBs titer (P<0.001).According to the above mentioned results, for a high risk group population such as medical students and residents, who are at continuous exposure to HBV, it is reasonable to determine the anti HBsAb response at one month post vaccination However, in order to confirm the persistence of immune protection, we strongly suggest detection of the anti-HBsAb titer at 5 – 10 years after the last inoculation.
Key words: Anti HBs, ELISA, vaccination, seroprotection rates
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