Volume-5 ~ Issue-3
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Abstract: Morphometric studies were conducted using eleven quantitative body parameters of six species of Puntius genus – Puntius chola, P. gelius, P. conchonius, P. sophore, P. sarana sarana and P. ticto ticto occurring from Assam, India, in order to study the morphometric variation and interrelationship among these species. All measurements were taken on a continuous scale using digital vernier caliper parallel to the anterior-posterior body axis except for the body depth that was taken perpendicular to the body axis between dorsal and ventral margins. The means of all measurements were standardized. A correlation study between total length vs. other morphometric parameters and a multivariate principal component analysis were conducted using MS Excel and bdpro32 software. The results obtained showed a significant correlation between total length and other morphometric parameters (p < 0.05) except for total length and pre-orbital length. The scores of PC1, PC2 and PC3 were found to be most similar for P. sarana sarana and P. gelius indicating these two species to be most closely related followed by P.ticto ticto , P. chola and P. conchonius, while the PC scores of P. sophore were the most dissimilar. The findings of this study will help in developing new strategies for conservation and breeding programmes of these species.
Keywords: Morphometric studies, Puntius genus, morphometric variation, interrelationship, principal component analysis.
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Abstract :Nutrient management is one of the most important factors in successful cultivation of plants. Biofertilizers can affect the quality and quantity of crop. Low phosphate solubility is one of the most important factors limiting the plant growth in various soils. Many microorganisms can enhance phosphate solubility, but little is known about the magnitude of their phosphorus solubilizing ability. The native populations of phosphate-solubilizing bacteria and fungi were studied in different rhizospheres soil samples obtained from banana plant and its effect on spinach plant (Amaranthus cruentus L.) in order to compare the results. The present study focuses on the phosphate-solubilizing capacity of bacteria and fungi in rhizospheres soil samples obtained from banana plant, revealing the dominance of Aspergillus species (234.12 mm) as major phosphate solubilizers, along with Bacillus subtilis (160.82 mm) followed by Pseudomonas aeruginosa 126.11, Penicillium sp., 99.02 and Micrococcus sp., 89. 4. In the present study potent solubilizers were identified as A. niger and B. subtilis. Hence an attempt was made to optimize the phosphate solubilization of the potential solubilizers at different pH with temperature. It is found out that both bacteria and fungi showed maximum phosphate solubilization at pH 3.0 with its specific temperature at 280 C and 370 C.
Keywords: Aspergillus, Bacillus, phosphates, Amaranthus cruentus, rhizospheres soil
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Abstract :In vitro transcorneal permeation of itraconazole from oil drops and ophthalmic ointments was studied using freshly excised goat and sheep corneas. Permeation of itraconazole from castor oil was found maximum and minimum with kardi oil formulation. The higher permeation of itraconazole from castor oil formulation could be attributed to the lower partition coefficient of drug between oil and aqueous phase. The addition of benzyl alcohol, a preservative, in oil drops, increased the permeation of itraconazole. Partition experiments indicated increased partitioning of itraconazole in the aqueous phase in the presence of benzyl alcohol. The addition of phenyl mercuric acetate, phenyl mercuric nitrate and thiomersal as preservative, in castor oil drop decreased the permeation of itraconazole. Corneal hydration was within the desired limit below 80 %. Permeation of itraconazole from ointment containing dissolved drug was higher than the ointment containing solid drug. In antifungal study, the castor oil formulation showed maximum zone of inhibition against Candida albicans. Stability study conducted at 400 C and 70% RH indicated the formulations as quite stable to ensure 2 years shelf life.
Keywords: - itraconazole, partition coefficient, permeation, preservative, stability.
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