iosr-JPBS   Volume-9 ~ Issue-3 ~ May-June 2014

Abstract: Ventilator-associated pneumonia (VAP) is difficult to diagnose, expensive to treat, and is associated with significant morbidity and mortality. The major part of VAP pathogenesis is the formation of a biofilm structure which also plays role in its resistance to treatment. A strategy to preventVAP isby removing the biofilm structure. The aim of this study is looking up the biofilm predictive protein of Enterobacter gergoviae (E. gergoviae) which is recognized by Secretory Immunoglobulin A (sIgA) as a mucosal immune response in VAP patient. This study used a tube adherence method for the detection of biofilms, protein profiling techniques with SDS PAGE and blotting for the evaluation of the antigen antibody response. The sIgAwere isolated from bronchial aspirate of VAP patient at ICU RSSA Malang. From two E. gergoviae samples, one sample biofilm positive (sample 1), and theother was biofilm negative (sample 2). The biofilm predictive protein was detected using SDS PAGE, and is discovered to be 15.13 kDa. The sIgA from sample 1 (p 1) reacted with protein band 15.13 kDa, 38.02 kDa, 45.71 kDa and 47.21 kDa whilesIgA from sample 2 (p 2) reacted with 38.02 kDa, 45.71 kDa, 47.21 kDa, and 50 kDa. This finding was predicted that protein MW 15.13 kDa played role in biofilm formation.
Keywords:VAP,biofilm, Enterobactergergoviae, sIgA.

Abstract: Āyurveda has its own specific biochemical approach towards fundamental elements of Universe. Absolute knowledge of evolutionary matter cannot be completing through visual perceptive method but can only be implicated. Contemporary science is based on laws of Physics and chemical reactions of the component.
As per modern discipline of physics and chemistry, physical and chemical properties are characteristic as per its electron configuration. Law of thermodynamics, law of redox reaction, laws of Acid-base reactions is based on analogous Quantum theory. A redox reaction is based on electron affinity of two compounds. Oxidations reactions are elevating free radicals produced by Oxidizing agents. Antioxidants are often relegating free radicals by reducing agents. Acid Base reactions also are explained on Hydrogen ion or on electron donor- acceptor formula. Thus, in modern science, electron configuration of the element forms foundation.
Āyurveda deals these aspects on the principles of Vaiśeshika philosophy of Padārtha and on the basis of Panca-Mahābhuta. Āyurveda explains biochemical process based essentially on Guṇa Theory that follows principle of Sāmānya- Viśeṣa. Āyurveda has emphasis on Panca-Mahābhuta as fundamental elements that can only be inferred in all the universal elements. Pancabhautika structures are established on foundation of Guṇa (attribute). Guṇa (attribute) is factor inherently associated vice versa with compound which is based on its Pancabhautika structure. Balance (equilibrium) of energy and function in an entity is to be balanced through elevating (Sāmānya) and relegating (Viśeṣa) of Guṇa (Attributes).

Abstract: The composition, abundance and distribution of the plankton of Kusalla reservoir was studied between June 2006 and May 2007. With the aim of assessing the responses of the plankton to seasonal and spatial changes. Plankton samples were collected monthly from six sampling stations using standard methods. Eighteen species of phytoplankton belonging to four taxa were recorded. Twenty species of zooplankton from four taxa were observed. There was significant seasonal and spatial variation (p=0.05) in the distribution of the phytoplankton and zooplankton. There was significant positive correlation (r = 0.7689) between the distribution and abundance of phytoplankton and zooplankton in Kusalla reservoir. Shannon-Weiner index of 5.17, 4.24 and 5.24 for stations 1,2 and 6 respectively indicate that these stations have a relatively high species diversity and therefore are not polluted. The distribution of the plankton shows that they are sensitive to changes in levels of nutrients and other interactions with one another and with other factors. As such the plankton can be good indicators of water quality.
Key Words: Composition, Distribution, Kusalla, Plankton

Abstract: Objective: To determine the frequency of the PROGINS polymorphism and the estrogen receptor b gene (ERβ) +1730 G/A polymorphism in infertile women with endometriosis and fertile women Subjects and methods: Case-control study included 50 women with endometriosis, and 24 fertile women as control. The PROGINS polymorphism and the ERβ gene + 1730 G/A polymorphism were identified by Restriction Fragment Length Polymorphism – Polymerase Chain Reaction (RFLP-PCR). Results: Genotypes A1A1, A1A2 and A2A2 (A2 representing the PROGINS polymorphism) of the progesterone receptor gene presented frequencies of 88%, 10 %and 2%, respectively, in the women with endometriosis. The allelic frequency of the PROGINS polymorphism was lower in women with endometriosis (P > 0.05) compared with healthy females in the patients with minimal/mild endometriosis (p = 0.978), 84.4 %, 12.5 % and 3.1 %, respectively, among the patients with moderate/severe endometriosis (p = 0.595); 94.4%, 5.6% and 0.0%, respectively, and 83.3%, 12.5 % and 4.2%, respectively, in the control group. Genotypes GG, GA and AA of the ERβ gene presented frequencies of 56%,34% and 10%, respectively, in the women with endometriosis (p < 0.05). In the control group; 91.8 % presented the normal homozygous genotype GG, 8.2% the GA heterozygous genotype and 0.0 % the homozygous mutated genotype AA. Conclusion: The data suggest that the estrogen receptor gene (ERβ) +1730 G/A polymorphism may be associated with risk of endometriosis. In contrast, the PROGINS polymorphism does not seem to modify the risk of developing endometriosis.
Key words: Endometriosis, polymorphism, PROGINS, estrogen receptor β.