Version-1 (March-April 2014)
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Abstract: In view of the neurotoxic effects induced by chlordecone an attempt is made to study the in vivo effect of this organic insecticide on rat brain based nitric oxide synthase (NOS) activity. Rats cerebellum, hypothalamus, midbrain, straiatum, hippocampus, cerebral cortex and medulla oblongate receiving sub-lethal and lethal doses of chlordecone showed decreased levels of their constitutive NOS activity and the changes were found to be statistically significant (p<0.001) over the control. Since cNOS activity in dependent on calcium / calmodulin, interaction of chlordecone with Ca²⁺/ CaM mediated events might be the reason for inhibition of cNOS activity of various brain parts of rats.
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[4] Bredt, D.S. and Snyder, S.H. 1989. Nitric oxide mediates glutamate - linked enhancement of cGMP levels in the cerebellum. Proc. Natl. Acad. Sci., USA. 86 : 9030-9033.
[5] Bredt, D.S. and Snyder, S.H. 1990. Isolation of nitric oxide synthatase, a calmodulin - requiring enzyme: Proc. Natl. Acad. Sci.,USA, 87 : 682 - 685.
[6] Chighizola, C. and Meroni, P. L. 2012. The role of environmental estrogens and autoimmunity. Autoimmunity – Reviews – Elsevier
[7] Connor, J.C.O., Frame, S.R and Ladics, G.S 2002. Evaluation of a 15-day screening assay using intact male rats for identifying antiandrogens. SOC. Toxicology.
[8] Desaiah, D. and Koch, R.B. 1975. Inhibition of ATPase activity in channel catfish by kepone its reduction product. Bull. Environ. Contam. Toxicol., 13 : 153-158.
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| Paper Type | : | Research Paper |
| Title | : | Effect of Growth Media and Incubation Time on the Culturability of Soil Bacteria |
| Country | : | Nigeria |
| Authors | : | C.B. Chikere, U. Udochukwu |
| : | 10.9790/3008-09210609 ![]() |
Abstract: The effect of growth medium and incubation time were investigated by determining the number of colonies that were visible on triplicate plates of three different media on daily intervals. The viable counts were significantly different between the three different media even after the first day and this continued for seven days. Colony counts on the three media reached their maximum on the third day of incubation. The use of Nutrient agar (NA) and Plate count agar (PCA) resulted in increasing counts with increasing incubation time respectively.
[2]. Bollmann, A., Lewis, K., Epstein, S S.; (2007): Incubation of Environmental Samples In A Diffusion Chamber Increases. The Diversity Of Recovered Isolates. Appl. Environ. Microbial. 73:6386-6390.
[3]. Buckley, D. H., Huangyutitham, V., Nelson, T. A., Rumberger, A.; (2006): Diversity of Planctomycetes in Soil In Relation To Soil History and Environmental Heterogeneity. Appl. Environ. Microbial. 72:4522-4531.
[4]. Eichorst, S. A., Brezenak, J. A., Schmidt, T. M.; (2007): Isolation and Characterization Of Soil Bacteria That Define Terriglobus Gen. Nov., In The PhyiumAcidobateria. Appl. Environ. Microbial. 73: 2708-2717.
[5]. Ferrai, B. C., Gillings, M. R.; (2009) : Cultivation of Fastidious Bacteria by Viability Staining and micromanipulation in a soil substrate membrane system. Appl. Environ. Microbiiol. 75: 33352-3354.
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Abstract: The biodegradability of petrol based engine oils produced by African Petroleum (AP) filling station was investigated for a period of twenty-eight (28) days. Two different samples of AP engine oils, Visco 2000-SAE20 (V20) and Super-V SAE 20 (S20)) were investigated. Soil from a mechanic workshop in Alakahia village near the University of Port Harcourt, Rivers State, Nigeria served as seed for the biodegradation. The study was carried out at room temperature (27-290C). The pH of the test systems ranged from 6.5 to 7.4. The counts on the first day were2.62x105 and 2.47 x105 for V20 and S20 respectively.Whereas the corresponding counts in 28th day were 2.70 x106 and 1.86 x106 in colony forming unit per milliliters (cfu/ml). The hydrocarbon utilizing bacteria (HUB) isolated include Bacillus, Citrobacter, Micrococcus, Proteus, Pseudomonas, and Vibrio. The physicochemical analysis showed that nitrate, phosphate, sulphate, biochemical oxygen demand, (BOD), oil and grease content and total organic carbon (TOC) decreased with time. The rate of biodegradation between the two engine oils was in accordance with the normal growth rate for a close system.
[2]. Anthony and Okoh (2006). Biodegradation Alternative in the Cleanup of Petroleum Hydrocarbon Pollutants.Biotechnol. Mol. Biol. 1: 38-50.
[3]. Chaillan F., Chaineau C. H. Point V., Saliot A., and Oudot J. (2006). Factors Inhibiting Bioremediation of Soil Contaminated with Weathered Oils and Drill Cuttings. Env. Pol 144: 255-265.
[4]. Chaineau C. H., Rougeux G., Yepremian C. and Oudot J. (2005). Effects of Nutrient Concentration on the Biodegradation of Crude Oil and Associated Microbial Populations in the Soil.Soil Biol. And Biochem.37: 1490-1497).
[5]. Cheesbrough M. (2006). District Laboratory Practice in Tropical Countries.McGraw Hill Publishers, New York.pp 143-156.
[6]. Fought J. M. and Westlake D. W. S. (1988). Degradation of Polycyclic Aromatic Hydrocarbon and Aromatic HeterocyclesByPseudomonas species. Can. J. Microbiol.34: 1135-1141.
