IOSR-JESTFT   Volume-4 ~ Issue-1 ~ May–June 2013

Abstract: Hydrocarbon degrading microorganisms play a major role in the environment.. The purpose of the present study was to enumerate of Aeromanas sp from oil contaminated soil and to study degrading capacity, emulcification activity and production of biosurfactant. The hydrocarbon substrate specificity test shown that diesel is also one of the best substrate for growth and emulsification of biosurfactant by Aeromonas. Among 6 strains of Aeromonas, frist strain(A1) shows maximum degradation rate at end of 168 hrs upto19.37% followed by other strains, about 75% of diesel was degraded by Aromonas over a period of 7days. Emulsification upto 75% by A1 followed by A2(63.75%) ,A3(57.5%), Biosurfactant production by A1 strain 0.064g/1 followed by other strains it represents a new type of biosurfactant with strong emulsifying ability.

Keywords: Aeromonas, Biosurfactant ,Diesel, Emulsify, Soil

[1]. Alexander. M. 1995. Biodegradation and bioremediation Chapter 2 New York Academic press.
[2]. Amikam Harounitz., Damid Gutnick and Eugene Roserberg. 1975. Sequential growth of bacteria on crude oil. Applied microbiology, 30: 10 – 19.
[3]. Apalijalahti. JHA. and Salkinoja Salonen M.S. 1984. Absorption pentachtorophenol (PCP) by bark chips and its role microbial PCP degradation. Microb. E.coli.10: 359 – 367.
[4]. Atlas. R. M. and R. Bartha. 1972. Degradation and mineralization of petroleum by two bacteria isolated from coastal waters. Biotechnol. Bioeng. 14:297-308.
[5]. Atlas. R.M. 1981. Fate of oil from two major oil spills. Environ. It 5: 33 – 38.,Microbial degradation of petroleum hydrocarbons, an environmental perspective. Microbial Rev. 45: 180 – 209.

Abstract: In artisanal and small-scale gold mine (ASGM) area of West Lombok, Indonesia, gold is recovered through a two-stage process of amalgamation and cyanidation. Tailing is discharged to land with no concern for contaminants in the tailings. Phytoremediation could cost-effectively replace traditional mercury remediation strategies. The objective of this study was to investigate the potential of six wild plant species (Lindernia crustacea (L.) F., Digitaria radicosa Miq., Zingiber purpurium Roxb., Paspalum conjugatum L., Cyperus kyllingia Endl., and Caladium bicolor L.) for phytoremediation of soil contaminated with mercury of gold cyanidation tailings. This study was conducted in a shade house located nearby a cyanidation processing facility of ASGM at Sekotong Distrik of West Lombok. Samples of tailings-uncontaminated soil were mixed with samples of cyanidation tailings with the proportion of 30%: 70% (w/w). Each of the six plant seedlings was planted in a plastic pot containing 15 kg of soil-tailings mixture. At harvest (9 weeks), shoots and roots were analyzed for mercury concentration In general, the results indicated that none of the plant species were identified as hyperaccumulator. Based on BCFs, TFs, and BAC, L.crustacea, D.radicosa, P. conjugatum and C. kyllingia can be used for phytoextraction of Hg since they were efficient to take up and translocate mercury from roots to shoots. On the other hand, Z. purpureum and C. bicolor can be used for phytostabilization of mercury.

Keywords –gold mine, mercury, phytoextraction, tailings

[1] C. Aspinall, Small-scale mining in Indonesia. International Institute for Environment and Development and the World Business Council for Sustainable Development, England, 2001.
[2] L.D.Hylander, D. Plath, C.R. Miranda, S. Lucke, J. Ohlander, and A.T.F. Rivera, Comparison of different gold recovery methods with regard to pollution control and efficiency. Clean 35, 2007, 52-61.
[3] P. Cordy, M. Veiga, I. Salih, S. Al-Saadi, S. Console, O. Garcia, L. Mesa, P. Velasquez-Lopez and M. Roeser, Mercury contamination from artisanal gold mining in Antioquia, Colombia: The world's highest per capita mercury pollution. Science of the Total Environment 410–411, 2011, 154–160.
[4] P.C. Velasquez-Lopez, M.M. Veiga, B. Klein, J.A. Shandro and K. Hall, Cyanidation of mercury rich tailings in artisanal and small-scale gold mining: identifying strategies to manage environmental risks in Southern Ecuador. Journal of Cleaner Production 19, 2011, 1125–1133.
[5] B.D. Krisnayanti, C.W.N. Anderson, W.H. Utomo, X. Feng, E. Handayanto, N. Mudarisna, H. Ikram and Khususiah, Assessment of environmental mercury discharge at a four-year-old artisanal gold mining area on Lombok Island, Indonesia. Journal of Environmental Monitoring 14, 2012. 2598-2607.
[6] US Environmental Protection Agency, Introduction to Phytoremediation, EPA/600/R-99/107 (2000)
[7] A.Zayed, E.H.A. Pilot-Smits, M. de Souza, Z.Q. Lin, and N. Terry, Remediation of selenium-polluted soils and waters by phytovolatilisation, in N. Terry and G. Banuelos (eds), Phytoremediation of Trace Elements (Ann Arbor Press, Michigan, 2000), 61-84.
[8] R.B. Meagher, C.L. Rugh, M.K. Kandasamy, G. Gragson, and N.J Wang, Engineering phytoremediation of mercury pollution in soil and water using bacterial genes. in N. Terry and G. Banuelos (eds), Phytoremediation of Contaminated Soil and Water (Lewis Publishers, USA, 2000), 201-219.
[9] I. Raskin, and B.D. Ensley, Phytoremediation of Toxic Metals: Using Plants to Clean Upthe Environment, (John Wiley & Sons, New York, 2000).
[10] J.W. Huckabee, F.S. Diaz, S.A. Janzen, and J. Solomon, Distribution of Mercury in Vegetation at Almanden, Spain, Environmental Pollution Serie A 30, 1983, 211-224

Abstract: The study aimed to know the effect of cement industry pollutant viz. SPM, RSPM on morphological as well as yield characteristics of groundnut plants grown at different distance from the cement industry at Kodinar, Gujrat, India. The groundnut plant near the industry showed significant deterioration in morphological characteristics. The study indicated that parameters reductions in groundnut correlated directly with particulate pollutant which led to lower yield at more polluted site. Increased concentration of cement dust pollutant causes in visible injuries like progressive decline in photosynthetic ability and closure of leaf stomata and this affect the growth and productivity of the plant.

Keywords- Cement industry, Growth parameter, Chlorophyll, Groundnut and yield.

[1] M.A. Addo, E.O. Darko, C.Gordon and B.J.B. Nyarko, Contamination of soils and loss of productivity of Cowpea (Vigna unguiculata L.) caused by cement dust pollution, Inter. J. Res. In Chem. and Env, 3(1), 2013, 272-282.

[2] M. Agrawal, B. Singh, M. Rajput, F. Marshall and J.N.B. Bell, Effect of air pollution on periurban agriculture: a case study, Environmental Pollution, 126, 2003, 323-329.

[3] A. Chauhan and P.C. Joshi, Effect of ambient air pollutants on wheat and mustard crops growing in the vicinity of urban and industrial areas, New York science J, 3(2), 2010, 52-60.
[4] M. Kampa and E. Castanas, Human health effect of air pollution, Environmental Pollution 151, 2008 362-367.

[5] T. Keller and H. Schwanger, Air pollution and ascorbic acid, European Journal of Forest pathology, 7, 1977, 338-350.

[6] S. Machlachlan and S. Zalik, Plastid structure, chlorophyll concentration and free amino-acid composition of a chlorophyll mutant of Barley, Can J. Bot, 40, 1963, 1053-1062.

[7] D.D. Pandey and S. Kumar S, Impact of cement dust pollution on biomass, chlorophyll, nutrients and grain characteristics of wheat, Environ Ecol 14, 1996, 872–875.

[8] S. Parthasarathy, M. Arunachalam, K. Natarajan, G. Oblisami and G. Rangaswami, Effect of cement dust pollution on certain physical parameter of maize crop and soils, Indian J. Environ. Hlth, 17, 1975, 114-120. [

9] G.J. Peirce, An effect of cement dust on orange trees, Plant World 13, 1910, 283-288.

[10] M.S.V. Prasad and J.A. Inamdar, Effect of cement kiln dust pollution on ground nut, Indian Bot Cont. 7(4), 1990, 159–162.

Abstract: Plant species used in the treatment of skin diseases among the indigenous communities of G. Madugula Mandalam, Visakhapatnam District was conducted between 2012-2013. Thirty three plant species belonging to 23 families were found to be used specifically in the treatment of various skin diseases.

KeyWords: Folklore treatment, Skin diseases, Tribal people, G.Madugula Mandalam, Visakhapatnam District

[1]. Anonymous. Medicinal plants in skin care, In: Central Institute of Medicinal and Aromatic plants, (CIMAP, Luck now, India), 1994, 425-430.

[2]. Bisht BS. Tribes of Uttarakhand: A study of education, health, hygiene and nutrition. Delhi: Kalpas Publication, 2006, pp. 236-267.

[3]. Chauhan N.S., Medicinal and Aromatic plants of Himachal Pradesh, 1999. Indus Publ. Co.NewDelhi.

[4]. De Silva T. Industrial utilization of medicinal plants in developing countries, In: Medicinal plants for forest conservation and healthcare, 1997. Edited by B. Odekar G., Bhat, KKS, B. Urlay J. Vantomme P. (Non wood forest products), No. 11, FAO, Rome, Italy: 38-48. [5]. Dwivedi S.N. and Pandey A. Ethnobotanical studies on wild and indigenous species of Vindhyan Plateau, 1999, 1. Herbaceous flora, J. Econ Taxon Bot.:143-150.

[6]. Dwivedi S.N. Ethnobotanical studies and conservational strategies of wild and Natural Resources of Rewa district, Madhya Pradesh, 2003. JECONTax Bot., (1): 233-244. 10

[7]. Haines H.H. The Botany of Bihar and Orissa 6 parts, 1921-1924. London, Reprinted 1961, Botanical Survey of India, Calcutta, 1-3.

[8]. Hooker J.D., (Hook F.) (Ed.). The flora of British India, 1872-1897. Reeve and Co. London. Reprinted 1973. Bishan Singh Mahindra Pal Singh, Dehradun and Periodical experts, Delhi: 1-72.

[9]. Kala C.R., Indigenous uses, population density and conservation of threatened medicinal plants in protected areas of the Indian Himalayas, 2005. Conserv Biol., (2): 368-378.

[10]. Kapur S.K. Review on ethno-medico plants for skin affections, 1991. India Drugs, (5): 210-223.