| Paper Type |
: |
Research Paper |
| Title |
: |
Low Dose Tcdd Affects Membrane Bound Ion Dependent Atpases
in Mice Liver |
| Country |
: |
India |
| Authors |
: |
Jyoti Jigyasi, Rahul Kundu |
 |
: |
10.9790/2402-0251519  |
Abstract: Present communication reports in vivo dose and duration dependent effects of a dioxin (2,3,7,8
TCDD) on few membrane bound ion dependent ATPases of mice hepatocytes. Two hypotheses were tested in
this study. Separate groups of mice were exposed to two very low sublethal doses (0.004 mg/kg bw, 0.04 mg/kg
bw) of TCDD by daily oral administration for 2, 4 and 6 days. Separate control groups were also maintained
which received only corn oil, the vehicle. The results indicated a significant exposure duration dependent
alterations in the membrane ATPases. No clear dose dependent effects were observed. The results suggested
that the observed alteration in the enzyme activity was possibly due to the oxidative stress generated by TCDD
on the membrane-bound ion-dependent ATPases. The results also indicated that the membrane bound
hydrophilic ATPases were indeed influenced by the hydrophobic TCDD. However, the effects were possibly
indirect through complex chain of reactions exhibited by the cells under in vivo conditions. ROS was suspected
to have played an important role in the alterations of membrane transport systems and permeability which
caused cellular injury, and thereby exhibiting exposure duration dependent effects.
Keywords: ATPases, Dose & Duration dependent effects, liver, mice ,TCDD
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