Abstract: An efficient method was developed for the simultaneous determination of sunset yellow and azorubine in carbonated orange flavor soft drink samples by high performance liquid chromatography (HPLC-UV-Vis). Affecting parameters on separation and detection of the dyes were investigated and optimized. For these dyes good linearity (0.25–50 mg L-1, > r2=0.99) were obtained. Limits of detection for both of the dyes were 0.1 mg L-1. The recoveries of the dyes ranged from 92 to 102%. Intra and inter-day precision expressed as relative standard deviation (RSD%) at 5.0 and 50 mg L-1 levels less than 8.0% were also achieved........
KeyWords: Synthetic dyes, High performance liquid chromatography, Sunset yellow, Azorubine, Carbonated orange flavor soft drinks.
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