Version-2 (Sep–Oct 2017)
- Citation
- Abstract
- Reference
- Full PDF
Paper Type | : | Research Paper |
Title | : | Cell Culture Technologies-A Short Review |
Country | : | India |
Authors | : | P.Praveen kumar || B.Mounika |
: | 10.9790/264X-03050115 |
Abstract: Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment.The cells may be removed from the tissue directly and disintegrated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line.The term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes)
[1] www.thermofisher.com/in/en/...cell-culture.../introduction-to-cell-culture.
[2] www.biologydiscussion.com/cell/cell-lines/cell-lines-types-nomenclature
[3] CA Gleaves, TF Smith, EA Shuster… - Journal of Clinical Microbiology…, 1985 vol-55, issue-2,Pages.221-227
[4] M Sittinger, J Bujia, N Rotter, D Reitzel, WW Minuth… - Biomaterials, 1996 - ElsevierVolume 17, Issue 3, 1996, Pages 237-242. [5] KE Hellstrom, I Hellstrom - Annual Reviews in Microbiology, 1970 - annualreviews.org volume 24 pages 373-398..
- Citation
- Abstract
- Reference
- Full PDF
Abstract: In this study, the uptake of Na+ and K+ into roots of salt tolerant rice cultivars (Oryza sativa L.cvs FR13A) and salt sensitive rice cultivar (BRRI dhan29) were measured using atomic absorption spectrophotometer. By means of inhibitor analyses the mechanisms for uptake and sequestration of Na+ in the salt-sensitive indica rice cv. BRRI dhan29 and in the salt-tolerant indica rice cv.FR13A mainly were detected. Lowest amount of Na+ was taken up into the roots, leaf sheath and leaf blade of FR13A than those of BRRI dhan29. In case of K+, highest amount of K+ was found in the roots, leaf sheath and leaf blade of FR13Afollowed by BRRI dhan29. The uptake.........
Keywords: Rice, salt stress, sodium and potassium uptake, selective and non-selective channels
[1] Blumwald E. 2000. Sodium transport and salt tolerance in plants. Current Opinion in Cell Biology 12, 431–434.
[2] Demidchik V, Davenport RJ and Tester M. 2002. Non-selective cation channels in plants. Annual Review of Plant Physiology and Plant Molecular Biology 53, 67–107...
[4] Fukuda A, Nakamura A, Tagiri A, Tanaka H, Miyao A, Hirochika H and Tanaka Y. 2004. Function, intracellular localization and the importance in salt tolerance of a vacuolar Na+/H+ antiporter from rice. Plant and Cell Physiology 45, 146–159.
[5] Golldack D, Su H, Quigley F, Kamasani UR, Munoz-Garay C, Balderas E, Popova OV, Bennett J, Bohnert HJ and Pantoja O. 2002. Characterization of a HKT-type transporter in rice as a general alkali cation transporter. The Plant Journal 31, 529–542
- Citation
- Abstract
- Reference
- Full PDF
Abstract: In this paper focused on the The medicinal plants GA and GU confirmed the presence of many phytoconstituents present in the ethanol extract showed a significant number of phytochemicals in them and the ethanol extract were used for further studies, The spectral studies of the plants also revealed the presence of many components in them. The study on in vitro antioxidant activity of medicinal plants also revealed the antioxidant potential of the plants. Hepatoprotective activity studies of the barks of GA and GU on paracetamol induced rats showed the significant protective activity.The ethanol extract of GA and GU extracts showed significant nephroprotective activity.In conclusion, the results of the present investigation infer that these plants (GA and GU) extracts possess potent antioxidant, Hepatoprotective and nephroprotective property, the former being probably responsible than the later. Thus, the extracts can be beneficial in treating liver and renal damages caused due to chemical exposure.
Keywords: Gmelina arborea, Ggrewia umbellifera, borntrager's test.
[1]. Activity of Five Selective Medicinal Plants. African Journal of Scientific Research, 2(1): 126–147.
[2]. Ashis G. Herbal folk remedies of Bankura and Medinipur districts, West Bengal. Indian Journal of Traditional Knowledge 2003; 2:393-396.
[3]. Baris O, Gulluce M, Sahin F, Ozer H, Kilic H, Ozkan H, Sokmen M and Ozbek T, 2006. Biological activities of the essential oil and methanol extract of Achillea Biebersteinii Afan. (Asteraceae). Turk. J. Biol., 30: 65–73.
[4]. Hibiscus sabdariffa leaves on hepatocelluar carcinoma cell line Hep 3B. Res. J.
[5]. Idris Torel, Haneli Ozbek, Remzi Erten, Ahmet Cihat Oner, Nureddin Cengiz and Orhan Yilmaz, 2009. Hepatoprotective and anti inflammatory activities of Plantago Major L. Indian Journal of Pharmacology, 41: 120–124.
- Citation
- Abstract
- Reference
- Full PDF
Abstract: The study assessed the effect of three pre-treatment techniques and three different growth media for breaking seed dormancy and in vitro germination in Adansonia digitata L., a multi-purpose and globally endangered forest tree. The seeds were pretreated with three different techniques (mechanical, thermal and chemical treatments). After pre-treatment, the seeds were allowed to germinate on three different types of growth media (Potting soil-PS, Murashige and Skoog's -MS medium and Paper-boat - PB). It was observed that hot water (100oC) treatment showed 88.33% of germination in potting soil. Whereas, the seeds treated with 98% H2SO4 for 24 hours showed 94.33% of germination with............
Keywords: Pre-treatment, Growth media, Seed dormancy, in vitro germination, Adansonia digitata
[1]. Al-Qarawi, A.A., Al-Damegh, M.A. and El-Mougy, S.A., 2003. Hepatoprotective influence of Adansonia digitata pulp. Journal of Herbs, Spices & medicinal plants, 10(3), pp.1-6.
[2]. Amusa, T.O., 2011. Effects of three pre-treatment techniques on dormancy and germination of seeds of Afzeliaafricana (Sm. Ex pers). Journal of Horticulture and forestry, 3(4), pp.96-103.
[3]. Bosch, C.H., Sié, K. and Asafa, B.A., 2004. Adansonia digitata L. PROTA (Plant Resources of Tropical Africa/Ressourcesvégétales de l'Afriquetropicale), Wageningen, Netherlands.
[4]. Buchmann, C., Prehsler, S., Hartl, A. and Vogl, C.R., 2010. The importance of baobab (Adansonia digitata L.) in rural West African subsistence—suggestion of a cautionary approach to international market export of baobab fruits. Ecology of Food and Nutrition, 49(3), pp.145-172..
- Citation
- Abstract
- Reference
- Full PDF
Abstract: Biosafety and antibiotic sensitivity ofmicroorganisms obtainedfrom domestic and restaurant wastewater was assessed. Wastewater samples were collected from different residential home and restaurant facility within Port Harcourt Metropolis. The Tyndallization test, total and faecal coliform counts and sensitivity to antibiotics were done using conventional methods. The total heterotrophic count for restaurant and domestic wastewater samples ranged from 1.06x106cfu/ml to 1.19x106cfu/ml and 1.0x104cfu/ml to 1.2x104cfu/ml for bacteria and fungi respectively. The coliform analysis revealed that fecal and total coliform had an MPN-index of 2.4x103cfu/100ml. The microflora obtained from the restaurant wastewater are Corynebacteriumsp, Hafniasp, Klebsiellasp, Staphylococcus sp, Bacillus sp............
Keywords: Antibiotics resistance, biosafety, coliform, environment, wastewater
[1]. Adebisi, S. O. and Fayemiwo, K. A. (2011).Physiochemical properties of industrial effluent in Ibadan, Nigeria.Electronic Journal of Environmental, Agriculture and Food Chemistry 10(3):2026-2031
[2]. Akoachere, J. T. K., Oben, P. M., Mbivnjo, B. S., Ndip, L. M., Nkwelang, G. and Nidp, R. N. (2008). Bacterial indicator of pollution of Douala Lagoon, Cameroon: public health implication. Africa Health Science 8(2): 85-89
[3]. Akpor, O. B. and Muchie, M. (2011).Environmental and public health implications of wastewater quality.African Journal of Biotechnology 10(13): 2379-2387.
[4]. Arora, U., Aggarwl, A. and Joshi, V. (2006). Fungal profile and susceptibility pattern in cases of Keratomycosis. Journal of Applied Science 8(1): 39- 41
[5]. Bukar, A., Uba, A. and Oyeyi, T. I. (2010). Occurrence of some pathogenic bacteria in some minimally and fully processed ready –to –eat foods in Kano metropolis, Nigeria. Africa Journal of Food Science 4(2):32-36.
- Citation
- Abstract
- Reference
- Full PDF
Abstract: Children in developing countries within complementary feeding window are challenged with nutrition related health problems predicated in part upon poor nutritional knowledge of caregivers. This study therefore, compared the proximate and mineral nutrients, empirically determined, of a proposed complementary food ('Tom Bran') with those in two proprietary kinds (Cerelac and Friso Gold Wheat) for nutrient adequacy for the infant sub-population. The empirical data obtained (with the use of standard protocols) were statistical compared at p≥ 0.05. The result obtained demonstrated that Tom Bran is statistically richer in macro-nutrients (crude protein, crude fat and crude fibre) and energy than in either Cerelac or Friso Gold Wheat, save proximate ash content that was highest in Cerelac............
Keywords: 'Tom Bran', Cerelac, Friso, Gold Wheat, Mineral Nutrients, Proximate Composition, Complementary Food,
[1] Akinola, O.O., Opreh, O.P., Hammed I.A. 2014. Formulation of local ingredient-based complementary food in South-west Nigeria. Journal of Nursing and Health Science 3( 6): 57-61
[2] Amah, G.H., Ehichioya, E.D., Ayodele, O.O., Adetona, M.O., Ananaba, E.D., Fakunle, V.O. 2016. Bioavailability of selested dietary elements in a locally formulated complementary food. IOSR Journal of Applied Chemistry, 9(11), 12-17.
[3] Anigo, K.M., Ameh, D.A., Ibrahim, S., Danbauchi, S.S. 2010. Nutrient composition of complementary food gruels formulated from malted cereals, soybeans and groundnut for use in North-Western Nigeria. African Journal of Food Science 4(3): 65-72.
[4] Association of Official Analytical Chemists (AOAC). 1980. Official methods of Analysis 13th edn. Association of Official Analytical Chemists. Washington DC.
[5] Association of Official Analytical Chemists (AOAC). 1990. Official methods of Analysis Method 954.02.. 15th ed. Association of Official Analytical Chemists. Washington DC.