Volume-3 ~ Issue-3

Abstract: Nitrogen and sulphur containing heterocycles have much more significance in drug , pharmaceutical and medicinal chemistry since from last four decades .Hence, the viscometric study of recently synthesized heterocycles viz. 1-(4-hydroxy)-S-triazino-3-p-chlorophenyl- thiourea (L1),1-(4-hydroxy)-S-triazino–3- allylthiourea (L2) and 1-(4-hydroxy)-S-triazino-3-t-butylturea (L3), were carried out at various percentage compositions of solvent to investigate effect of structure , group on S-triazinothiourea, The data and result obtained during this investigation is useful in pharmokinetics and pharmodynamics. Taking all these things into consideration this research work was carried out.
Keywords: 1-(4-Hydroxy)-S-triazino-3-substitutedthiourea, dioxane-water, viscometric study.

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Abstract: The structural properties of N'-[(E)-(5-chloro-2-hydroxyphenyl)methylidene]acetohydrazide (NClHMAH) have been investigated by spectro-analytical and computational studies. ChemAxon and HyperChem 7.5 tools were employed to generate energy parameters, heat of formation, dipole moment values. Molecular orbital calculations were performed with quantum mechanics based semi empirical Parametric Method 3 (PM3). The compound NClHMAH was characterized by elemental analyses, IR, 1H – NMR, 13C – NMR, DEPT and Mass spectral data. The equilibrium studies were carried out to determine the dissociation constants of NClHMAH in 70% v/v DMF – water medium at 303 K and 0.1 M (KNO3) ionic strength adopting pH-metric technique. The dissociation of two protons is evident from these studies indicating it as a dibasic acid. The microbial studies affirmed moderate antibacterial activity of NClHMAH against Staph. Aureus and the compound is resistant to E.Coli. while its copper complex has exhibited enhanced anti – bacterial activity for both the cultures.
Keywords : N'-[(E)-(5-chloro-2-hydroxyphenyl)methylidene]acetohydrazide(NClHMAH), computational studies, equilibrium studies,mass, IR, 1H – NMR, 13C – NMR, DEPT.

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Abstract: High efficiency, cost effectiveness and short run time are the basic requirements of high-speed chromatographic separations. To fulfill these requirements, an advanced separation technique, ultra-performance liquid chromatography (UPLC), has shown promising developments. A rapid, specific, sensitive, and precise reverse-phase UPLC method was developed for the determination of antiparkinson drug and its impurities. In this work, a new gradient reverse phase chromatographic method using UPLC for the determination was done for active pharmaceutical ingredient in the presence of degradation products, and its process related impurities.The chromatographic separation was achieved on a Waters Acquity BEH C18 column (50 mm, i.d., 2.1 mm, 1.7 μm) within a short runtime of 4 minutes using a mobile phase of 5 mM potassium phosphate and acetonitrile, at a flow rate of 0.5 mL/min at an ambient temperature. Forced degradation studies were also performed for antiparkinson bulk drug samples to demonstrate the stability-indicating power of the UPLC method. Linearity range was LOQ-200% with respect to 100 μg mL-1 of active pharmaceutical ingredient for related impurities; Linearity range was 40-160% with respect to 10 μg mL-1of active pharmaceutical ingredient for assay calculated by 5 determinations. Comparison of system performance with conventional high-performance liquid chromatography was made with respect to analysis time, efficiency, and sensitivity. The method is validated according to the ICH guidelines and is applied successfully for the quantitative determination of antiparkinson drug and its related impurities.
Keywords :Ultra performance liquid chromatography (UPLC), Antiparkinson drugs, Validation and quantification

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Abstract: Soil and vegetation samples collected from the vicinities of wood processing industries from selected parts of Delta State, Nigeria were analyzed for their metal levels and physicochemical properties inorder to assess the impact of the industries on the surrounding environment. The pH values ranged from 4.9 to 6.0 indicating that the soils were acidic. The electrical conductivity values ranged from 54.80 to 75.90uScm which is suggestive of significant concentration of soluble inorganic salts. The total organic carbon and total nitrogen ranging from 0.25 to 0.45% and from 0.11 to 0.69% respectively showed some presence of degradable and compostable materials as well as organic matters in the soils. The cation exchange capacity which ranged from 1.23 to 3.95cmolkg-1 indicated the low capacity of the soils to adsorb metals. The mean levels of the heavy metals in the soils ranged from 12.69 to 272 mgkg-1 with abundance trend of Fe > Mn > Cu > Zn > As > Cr > Pb > Cd while those of the vegetation ranged from 4.17 to 47.10mgkg-1 with abundance trend of Fe > Mn > Zn > Cu > Cr > Pb > As. The metal levels in both the soil and vegetation samples in all the sites were significantly higher than the levels obtained in the control site which is suggestive of significant contribution of the industries to these elevated levels. The contamination/pollution index values of the metals in the soil samples showed generally contamination levels for all the metals except copper which showed moderate pollution. The soil plant transfer values indicate medium, accumulation for all the metals. Although the overall results showed evidence of contamination of the soils by heavy metals, the present levels do not pose any environmental risk or health hazard.
Keywords : Heavy metals; Physicochemical characteristics; pollution; soils; vegetation; Wood processing

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